LOCUS       sequence-104839-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (lambda 
            light chain) without variable regions. Enables Colony-PCR screening 
            of false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/lambda
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaggtatctt ttaaaccctt ttttaggtgt tgtgaaaacc accgctaatt caaagcaaat
       61 ggactggacc tggaggatcc tcttcttggt ggcggccgcc acaggcgcgc actccgaatt
      121 cgatatcctc gaggctagca cacagagccc atccgtcttc cccttgaccc gctgctgcaa
      181 aaacattccc tccaatgcca cctccgtgac tctgggctgc ctggccacgg gctacttccc
      241 ggagccggtg atggtgacct gggacacagg ctccctcaac gggacaacta tgaccttacc
      301 agccaccacc ctcacgctct ctggtcacta tgccaccatc agcttgctga ccgtctcggg
      361 tgcgtgggcc aagcagatgt tcacctgccg tgtggcacac actccatcgt ccacagactg
      421 ggtcgacaac aaaaccttca gcgtctgctc cagggacttc accccgccca ccgtgaagat
      481 cttacagtcg tcctgcgacg gcggcgggca cttccccccg accatccagc tcctgtgcct
      541 cgtctctggg tacaccccag ggactatcaa catcacctgg ctggaggacg ggcaggtcat
      601 ggacgtggac ttgtccaccg cctctaccac gcaggagggt gagctggcct ccacacaaag
      661 cgagctcacc ctcagccaga agcactggct gtcagaccgc acctacacct gccaggtcac
      721 ctatcaaggt cacacctttg aggacagcac caagaagtgt gcagattcca acccgagagg
      781 ggtgagcgcc tacctaagcc ggcccagccc gttcgacctg ttcatccgca agtcgcccac
      841 gatcacctgt ctggtggtgg acctggcacc cagcaagggg accgtgaacc tgacctggtc
      901 ccgggccagt gggaagcctg tgaaccactc caccagaaag gaggagaagc agcgcaatgg
      961 cacgttaacc gtcacgtcca ccctgc
//
