LOCUS       sequence-104843-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MXS_chaining vector with 2 cassettes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: rtTA and TagBFP cassettes
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    9..233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(263..373)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 ttggtcgacc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
       61 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
      121 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
      181 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagctcgaca
      241 gatccttact tagttacccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag
      301 gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat
      361 cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc aggccacata
      421 tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg aacagctcaa
      481 ttgcttgtct cagaagtggg ggcatagaat cggtggtagg tgtctctctt tcctcttttg
      541 ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc acggcggaca
      601 gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg agctga
//
