LOCUS       sequence-104844-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (lambda 
            light chain) without variable regions. Enables Colony-PCR screening 
            of false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/lambda
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    425..869
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        902..948
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        940..958
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccgctaat tcaaagcaat atggcctgga tgatgcttct cctcggactc cttgcttatg
       61 gatccggagt cgactctgaa ttcgatatcc tcgagctagg tcagcccaag gcggcgccct
      121 cggtcactct gttcccgccc tcctctgagg agcttcaagc caacaaggcc acactggtgt
      181 gtctcataag tgacttctac ccgggagccg tgacagtggc ctggaaggca gatagcagcc
      241 ccgtcaaggc gggagtggag accaccacac cctccaaaca aagcaacaac aagtacgcgg
      301 ccagcagcta tctgagcctg acgcctgagc agtggaagtc ccacagaagc tacagctgcc
      361 aggtcacgca tgaagggagc accgtggaga agacagtggc ccctacagaa tgttcatagc
      421 taggagcagg tttccccaat gacacaaaac gtgcaacttg aaactccgcc tggtctttcc
      481 aggtctagag gggtaacact ttgtactgcg tttggctcca cgctcgatcc actggcgagt
      541 gttagtaaca gcactgttgc ttcgtagcgg agcatgacgg ccgtgggaac tcctccttgg
      601 taacaaggac ccacggggcc aaaagccacg cccacacggg cccgtcatgt gtgcaacccc
      661 agcacggcga ctttactgcg aaacccactt taaagtgaca ttgaaactgg tacccacaca
      721 ctggtgacag gctaaggatg cccttcaggt accccgaggt aacacgcgac actcgggatc
      781 tgagaagggg actggggctt ctataaaagc gctcggttta aaaagcttct atgcctgaat
      841 aggtgaccgg aggtcggcac ctttcctttg caattactga ccctatgaat acactgactg
      901 tttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgactc actataggag
      961 ggccaccatg aagaaacctg aactgacagc aacttctgtt gagaagtttc tcattgaaaa
     1021 atttgattct gtttctgatc tcatgcagct gtctgaaggt gaagaaagca gagcctttt
//
