LOCUS       sequence-10485-e        1737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Lin CW, Jao CY, Ting AY
  TITLE     Genetically encoded fluorescent reporters of histone methylation in 
            living cells.
  JOURNAL   J Am Chem Soc. 2004 May 19. 126(19):5982-3.
  PUBMED    15137760
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2004 May 19. 126(19):5982-3."
COMMENT     Sequence Label: pcDNA3-K27 histone methylation reporter
FEATURES             Location/Qualifiers
     source          1..1737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..15,16..18,19..531)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             join(979..981,982..984,985..1695)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1696..1716
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gataaggatc cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
       61 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      181 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      301 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      421 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
      481 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
      541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      601 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
      661 gatcacatgg tcctgctgga gttcgtgacc gccgcccgca tgctcccagt cgatctagtg
      721 tacgcggctg agaaaatcat ccaaaagcgc gttaagaagg gcgtcgtgga gtaccgtgtc
      781 aagtggaagg gctggaacca gcgctacaac acctgggaac cggaggtaaa catcctggat
      841 cgccgcctca tcgacatcta cgaacaaacg aacaaatccg ggtctacatc tggatctggg
      901 aagccgggtt ctggtgaggg ttctgcccgg acggccgcag cgcgcaaaag cgctccagct
      961 acgggggggg tggagctcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1021 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1081 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1141 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
     1201 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1261 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1321 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1381 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1441 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1501 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1561 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     1621 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1681 gacgagctgt acaagccaaa aaagaagaga aaggtggaag acgcttaaga attccgg
//
