LOCUS       sequence-104861-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-FKBP3; ind. FRB-mCH-Mad1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB160
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           119..145
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           171..212
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     287..306
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             353..631
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     regulatory      644..653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
       61 agaaactggg cttgtcgaga cagagaagac tcttgcgttt cggtcgactt ctagagctct
      121 ggaggcttgc tgaaggctgt atgctagaga cgtacagatg cgtctcacag gacacaaggc
      181 ctgttactag cactcacatg gaacaaatgg ccgaattcgg cgctagctgc tgatatcgca
      241 tacgcgtgga ccagataggc acctattggt cttactgaca tccactttgc ctttctctcc
      301 acaggtgtcg gtaccgcggg cccgggatcc accggtcacc atggcttcta gaatcctctg
      361 gcatgagatg tggcatgaag gcctggaaga ggcatctcgt ttgtactttg gggaaaggaa
      421 cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct atgatggaac ggggccccca
      481 gactctgaag gaaacatcct ttaatcaggc ctatggtcga gatttaatgg aggcccaaga
      541 gtggtgcagg aagtacatga aatcagggaa tgtcaaggac ctcctccaag cctgggacct
      601 ctattatcat gtgttccgac gaatctcaaa gactagtccg gtcgccacca tggtgagcaa
      661 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttc
//
