LOCUS       sequence-10487-e        4257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, 
            Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA
  TITLE     Synthetic biology: engineering Escherichia coli to see light.
  JOURNAL   Nature. 2005 Nov 24. 438(7067):441-2.
  PUBMED    16306980
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2005 Nov 24. 438(7067):441-2."
COMMENT     Sequence Label: pCph8
FEATURES             Location/Qualifiers
     source          1..4257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(6..24,25..30,31..47,48..53,54..79)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             89..100
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2354..2440
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2605..3193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2685..2704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3281..3375)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3396..4055)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3970..3989
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4056..4158)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4224..4242
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtacccatg gccaccaccg
      121 tacaactcag cgaccaatcc ctccgtcagc tagaaaccct cgccatccac accgcccacc
      181 tgattcagcc ccacggttta gtggtggtcc tgcaggaacc agacctcacc atcagccaaa
      241 ttagcgccaa ctgcaccggc attttagggc gatcgccaga ggatttgttg ggcagaaccc
      301 taggggaagt gtttgatagc tttcagattg atcccatcca gagtcgccta acggccggac
      361 aaatcagcag cctcaacccc agtaaacttt gggcgcgggt catgggggac gactttgtca
      421 tttttgacgg ggtttttcat cgcaacagtg acggtttatt ggtatgtgaa ctagagccag
      481 cctacacttc cgataatctg cccttcctcg gtttttatca catggccaac gctgccctga
      541 atcggttgcg ccaacaagct aatctacggg atttctacga tgttattgtc gaagaagtcc
      601 gccgtatgac tggctttgac cgggtgatgc tataccgctt tgatgaaaat aaccacggtg
      661 atgtcattgc cgaagataaa cgggatgata tggaacccta tttgggcctg cactatcccg
      721 aatcggatat tccccaaccc gcccgtcggc tatttatcca caaccccatt cgagtaattc
      781 ccgatgttta tggtgtggcg gtgcccctga ccccagcggt taaccccagc accaaccgag
      841 cggtggattt aacagaatcc attctgcgca gtgcgtacca ttgccacttg acctatctga
      901 aaaatatggg ggtaggagcg tctttaacca tttccctaat taaggacggc catctctggg
      961 ggctcattgc ctgccaccat caaaccccca aagtaattcc ctttgaactg cgtaaagcct
     1021 gcgaattttt tggtcgggtg gtgtttagca acatttccgc ccaggaagat acggaaacct
     1081 tcgattaccg ggtgcagttg gcggagcatg aagcggtttt attggacaaa atgaccacgg
     1141 cggcggattt tgtcgaagga ttaactaatc atcccgatcg cctgttggga ttaacgggct
     1201 cccagggggc ggccatttgc tttggggaaa aattgatttt agtaggggaa accccggacg
     1261 agaaagcagt gcaatattta ctgcaatggt tggagaatcg ggaagtgcaa gacgttttct
     1321 tcacctcttc cctctcacaa atttatcctg atgcagtgaa ttttaaatcc gtggccagtg
     1381 gcttattggc cattcccatt gcccgtcaca actttttgct ctggtttcgc cctgaagtgt
     1441 tgcaaacggt taattggggc ggtgacccaa atcatgctta cgaagctacc caggaagacg
     1501 gtaaaatcga gctccatccc cgccaatcct ttgacctctg gaaagaaatt gtccgactcc
     1561 aatctttgcc ctggcaatcg gtggaaatcc aaagtgccct ggccctgaaa aaggcgatcg
     1621 tcaacctcat tttgcgccag gcagaagaat tgcatatggc ggctggtgtt aagcaactgg
     1681 cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg ccgctgacgc
     1741 gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca gaatcgatca
     1801 ataaagatat cgaagagtgc aacgccatca ttgagcagtt tatcgactac ctgcgcaccg
     1861 ggcaggagat gccgatggaa atggcggatc ttaatgcagt actcggtgag gtgattgctg
     1921 ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc attgaagtga
     1981 aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac gccgcccgtt
     2041 atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc tggttccagg
     2101 tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc cagccgtttg
     2161 tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca attgtgcagc
     2221 gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg ggcgggcttt
     2281 ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcatgaca aaagaagggt
     2341 aatctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2401 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggg
     2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3241 aaactcacgt taagggattt tggtcatgac tagtgcttgg attctcacca ataaaaaacg
     3301 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     3361 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
     3421 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga
     3481 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     3541 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     3601 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     3661 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     3721 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     3781 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     3841 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     3901 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     3961 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     4021 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     4081 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     4141 cctcttacgt gccgatcaac gtctcatttt cgccagatat cgacgtctaa gaaaccatta
     4201 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcacc
//
