LOCUS       sequence-104871-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to characterize the promoter activities of PTet for dCas9 and 
            sgRNA response functions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PTet-YFP
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             122..745
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(195..214)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
       61 gcagctggca cgacaggttt cccgactgga aagcgggcag tgataatcca ggaggaaaaa
      121 aatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      181 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      241 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      301 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      361 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      421 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      481 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      541 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      601 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      661 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      721 acttaaatgt gaaagtgggt cctaataatt ggtaacgaat cagacaattg acggctcgag
      781 ggagtagcat ag
//
