LOCUS       sequence-104879-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. CenpB-GFP-Halo + mCherry-DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM
  TITLE     Localized light-induced protein dimerization in living cells using a
            photocaged dimerizer.
  JOURNAL   Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475.
  PUBMED    25400104
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-17"; volume: "5:5475. doi"; pages: " 
            10.1038/ncomms6475"
COMMENT     Sequence Label: pERB221
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             236..709
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
       61 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
      121 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
      181 ccgccactcc accggcggca tggacgagct gtataaggcc ggcctggagt ctagaatcag
      241 tctgattgcg gcgttagcgg tagatcgcgt tatcggcatg gaaaacgcca tgccgtggaa
      301 cctgcctgcc gatctcgcct ggtttaaacg caacacctta aataaacccg tgattatggg
      361 ccgccatacc tgggaatcaa tcggtcgtcc gttgccagga cgcaaaaata ttatcctcag
      421 cagtcaaccg ggtacggacg atcgcgtaac gtgggtgaag tcggtggatg aagccatcgc
      481 ggcgtgtggt gacgtaccag aaatcatggt gattggcggc ggtcgcgttt atgaacagtt
      541 cttgccaaaa gcgcaaaaac tgtatctgac gcatatcgac gcagaagtgg aaggcgacac
      601 ccatttcccg gattacgagc cggatgactg ggaatcggta ttcagcgaat tccacgatgc
      661 tgatgcgcag aactctcaca gctattgctt tgagattctg gagcggcggg aacaaaaact
      721 catctcagaa gaggatc
//
