LOCUS       sequence-104919-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Const. Halotag-GFP-Nuf2 + mCherry-DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM
  TITLE     Localized light-induced protein dimerization in living cells using a
            photocaged dimerizer.
  JOURNAL   Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475.
  PUBMED    25400104
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-17"; volume: "5:5475. doi"; pages: " 
            10.1038/ncomms6475"
COMMENT     Sequence Label: pERB215
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(183..212)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(213..686)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt
       61 ggcagaggga aaaagatctc agtggtattt gtgagccagg gcattggcca caccagccac
      121 caccttctga taggcagcct gcacctgagg agtgaattgc ggccgcctcg agcgcttaat
      181 taaagatcct cttctgagat gagtttttgt tcccgccgct ccagaatctc aaagcaatag
      241 ctgtgagagt tctgcgcatc agcatcgtgg aattcgctga ataccgattc ccagtcatcc
      301 ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg cgtcagatac
      361 agtttttgcg cttttggcaa gaactgttca taaacgcgac cgccgccaat caccatgatt
      421 tctggtacgt caccacacgc cgcgatggct tcatccaccg acttcaccca cgttacgcga
      481 tcgtccgtac ccggttgact gctgaggata atatttttgc gtcctggcaa cggacgaccg
      541 attgattccc aggtatggcg gcccataatc acgggtttat ttaaggtgtt gcgtttaaac
      601 caggcgagat cggcaggcag gttccacggc atggcgtttt ccatgccgat aacgcgatct
      661 accgctaacg ccgcaatcag actgattcta gactccaggc cggccttata cagctcgtcc
      721 atgccgccgg tggagt
//
