LOCUS       sequence-10492-e        7871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Srinivasan A, McClellan AJ, Vartikar J, Marks I, Cantalupo P, Li Y, 
            Whyte P, Rundell K, Brodsky JL, Pipas JM
  TITLE     The amino-terminal transforming region of simian virus 40 large T 
            and small t antigens functions as a J domain.
  JOURNAL   Mol Cell Biol. 1997 Aug . 17(8):4761-73.
  PUBMED    9234732
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1997 Aug . 17(8):4761-73."
COMMENT     Sequence Label: pRSVBneoT
FEATURES             Location/Qualifiers
     source          1..7871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..50
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     34..50
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        371..632
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(644..660)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(645..661)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             join(708..953,1300..3180)
                     /codon_start=1
                     /product="SV40 (simian virus 40) large T antigen"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     intron          954..1299
                     /label=large T intron
                     /note="SV40 (simian virus 40) large T antigen intron"
     polyA_signal    3203..3337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3240..3259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3294..3313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(3375..3393)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3376..3393)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3411..3427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3411..3427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3424..3446)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3435..3451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3459..3465,3466..3483,3484..3489))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3504..3525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3642..3659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3813..4401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3893..3912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4572..5363,5364..5432))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5195..5214
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5433..5537)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5605..5623
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5661..5683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             6320..7123
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6383..6402)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6993..7012
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7130..7178
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      complement(7236..7691)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7373..7394)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7585..7604
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7832..7848
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7855..7871,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(7855..7871,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcaccg cggtggcggc cgctctagaa ctagtggatc gatcccccta
       61 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
      121 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
      181 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      241 gatgtacggg ccagatatac gcgtatctga ggggactagg gtgtgtttag gcgaaaagcg
      301 gggcttcggt tgtacgcggt taggagtccc ctcaggatat agtagtttcg cttttgcata
      361 gggaggggga aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
      421 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      481 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      541 ccactgaatt ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg
      601 ccatttgacc attcaccaca ttggtgtgca cctccaagct tatcgatacc gtcgacctcg
      661 agggggggcc cggtaccccc taggcttttg caaaaagctt tgcaaagatg gataaagttt
      721 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgcctggg
      781 ggaatattcc tctgatgaga aaggcatatt taaaaaaatg caaggagttt catcctgata
      841 aaggaggaga tgaagaaaaa atgaagaaaa tgaatactct gtacaagaaa atggaagatg
      901 gagtaaaata tgctcatcaa cctgactttg gaggcttctg ggatgcaact gaggtatttg
      961 cttcttcctt aaatcctggt gttgatgcaa tgtactgcaa acaatggcct gagtgtgcaa
     1021 agaaaatgtc tgctaactgc atatgcttgc tgtgcttact gaggatgaag catgaaaata
     1081 gaaaattata caggaaagat ccacttgtgt gggttgattg ctactgcttc gattgcttta
     1141 gaatgtggtt tggacttgat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     1201 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     1261 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     1321 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     1381 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     1441 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     1501 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     1561 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     1621 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     1681 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     1741 tatttgatgt atagtgcctt gactagagat ccattttctg ttattgagga aagtttgcca
     1801 ggtgggttaa aggagcatga ttttaatcca gaagaagcag aggaaactaa acaagtgtcc
     1861 tggaagcttg taacagagta tgcaatggaa acaaaatgtg atgatgtgtt gttattgctt
     1921 gggatgtact tggaatttca gtacagtttt gaaatgtgtt taaaatgtat taaaaaagaa
     1981 cagcccagcc actataagta ccatgaaaag cattatgcaa atgctgctat atttgctgac
     2041 agcaaaaacc aaaaaaccat atgccaacag gctgttgata ctgttttagc taaaaagcgg
     2101 gttgatagcc tacaattaac tagagaacaa atgttaacaa acagatttaa tgatcttttg
     2161 gataggatgg atataatgtt tggttctaca ggctctgctg acatagaaga atggatggct
     2221 ggagttgctt ggctacactg tttgttgccc aaaatggatt cagtggtgta tgacttttta
     2281 aaatgcatgg tgtacaacat tcctaaaaaa agatactggc tgtttaaagg accaattgat
     2341 agtggtaaaa ctacattagc agctgctttg cttgaattat gtggggggaa agctttaaat
     2401 gttaatttgc ccttggacag gctgaacttt gagctaggag tagctattga ccagttttta
     2461 gtagtttttg aggatgtaaa gggcactgga ggggagtcca gagatttgcc ttcaggtcag
     2521 ggaattaata acctggacaa tttaagggat tatttggatg gcagtgttaa ggtaaactta
     2581 gaaaagaaac acctaaataa aagaactcaa atatttcccc ctggaatagt caccatgaat
     2641 gagtacagtg tgcctaaaac actgcaggcc agatttgtaa aacaaataga ttttaggccc
     2701 aaagattatt taaagcattg cctggaacgc agtgagtttt tgttagaaaa gagaataatt
     2761 caaagtggca ttgctttgct tcttatgtta atttggtaca gacctgtggc tgagtttgct
     2821 caaagtattc agagcagaat tgtggagtgg aaagagagat tggacaaaga gtttagtttg
     2881 tcagtgtatc aaaaaatgaa gtttaatgtg gctatgggaa ttggagtttt agattggcta
     2941 agaaacagtg atgatgatga tgaagacagc caggaaaatg ctgataaaaa tgaagatggt
     3001 ggggagaaga acatggaaga ctcagggcat gaaacaggca ttgattcaca gtcccaaggc
     3061 tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc ataccacatt
     3121 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3181 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3241 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3301 gtccaaactc atcaatgtat cttatcatgt ctggatcctc tagagtcgac ctgcaggcat
     3361 gcaagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg
     3421 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3481 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3541 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3601 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3961 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4021 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4081 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4141 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4201 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4261 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4321 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4381 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4441 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4501 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4561 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4621 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4681 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4741 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4801 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4861 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4921 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4981 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5041 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5101 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5161 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5221 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5281 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5341 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5401 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5461 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5521 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     5581 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     5641 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     5701 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     5761 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     5821 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     5881 ttcaggctac gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     5941 ctggcgaagg ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     6001 tcacgacgtt gtaaaacgac ggccagggcc agtgaattct cgagcagtgt ggttttgcaa
     6061 gaggaagcaa aaagcctctc cacccaggcc tggaatgttt ccacccaatg tcgagcagtg
     6121 tggttttgca agaggaagca aaaagcctct ccacccaggc ctggaatgtt tccacccaat
     6181 gtcgagcaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
     6241 gggcggcgcg gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
     6301 gagcgaccct gcagccaata tgggatcggc cattgaacaa gatggattgc acgcaggttc
     6361 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6421 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6481 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6541 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6601 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6661 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6721 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6781 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6841 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6901 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6961 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7021 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7081 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaggggatc ggcaataaaa
     7141 agacagaata aaacgcacgg gtgttgggtc gtttgttcgg atctatgcgg tgtgaaatac
     7201 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt
     7261 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7321 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7381 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7441 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7501 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7561 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7621 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7681 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     7741 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     7801 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     7861 gactcactat a
//
