LOCUS       sequence-104922-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^549-718.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB174
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           128..154
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           180..221
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     296..315
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             362..640
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     regulatory      653..662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag
       61 gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc ggtcgacttc
      121 tagagctctg gaggcttgct gaaggctgta tgctagagac gtacagatgc gtctcacagg
      181 acacaaggcc tgttactagc actcacatgg aacaaatggc cgaattcggc gctagctgct
      241 gatatcgcat acgcgtggac cagataggca cctattggtc ttactgacat ccactttgcc
      301 tttctctcca caggtgtcgg taccgcgggc ccgggatcca ccggtcacca tggcttctag
      361 aatcctctgg catgagatgt ggcatgaagg cctggaagag gcatctcgtt tgtactttgg
      421 ggaaaggaac gtgaaaggca tgtttgaggt gctggagccc ttgcatgcta tgatggaacg
      481 gggcccccag actctgaagg aaacatcctt taatcaggcc tatggtcgag atttaatgga
      541 ggcccaagag tggtgcagga agtacatgaa atcagggaat gtcaaggacc tcctccaagc
      601 ctgggacctc tattatcatg tgttccgacg aatctcaaag actagtccgg tcgccaccat
      661 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      721 gcacatggag ggctccgtga ac
//
