LOCUS       sequence-104941-         509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-ACTLg
FEATURES             Location/Qualifiers
     source          1..509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    154..178
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(222..243)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttcttatat gctcaacaca tgagcgaaac cctataagaa ccctaattcc cttatctggg
       61 aactactcac acattattct ggagaaaaat agagagagat agatttgtag agagagactg
      121 gtgatttttg cggactctag catggccgct tattccactt tgtacaagaa agctgggtcc
      181 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      241 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      301 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      361 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      421 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      481 cccaggatgt tgccgtcctc cttgaagtc
//
