LOCUS       sequence-104949-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 
            500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: mCherry-miniSOG-C-Src-7
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             246..566
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    689..810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(726..745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     780..799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
       61 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      121 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      181 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
      241 gcggcatgga gaagagcttc gtgatcaccg acccccgcct gcccgacaac cccatcatct
      301 tcgccagcga cggcttcctg gagctgaccg agtacagcag agaggagatc ctgggccgca
      361 acggccgctt cctgcagggc cccgagaccg accaggccac cgtgcagaag atccgcgacg
      421 ccatccgcga ccagcgcgag atcaccgtgc agctgatcaa ctacaccaag agcggcaaga
      481 agttctggaa cctgctgcac ctgcagccca tgcgcgacca gaagggcgag ctgcagtact
      541 tcatcggcgt gcagctggac ggctaaagcg gccgcgactc tagatcataa tcagccatac
      601 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      661 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      721 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      781 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattt
//
