LOCUS       sequence-10495-e        6979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 6979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
COMMENT     Sequence Label: pAAV-mCAMK-RFP
FEATURES             Location/Qualifiers
     source          1..6979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     promoter        234..1523
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     intron          1587..2062
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     2144..2160
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2144..2160
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      2192..2201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2198..2875
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(2328..2346)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(2378..2398)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2596..2615
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2790..2813
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(2918..2936)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2919..2936)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2956..2973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2962..3073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3764..4277
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3851..3870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4061..4082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4758..4777)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4877..4899
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4937..4955)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5023..5127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5128..5196,5197..5988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5346..5365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6159..6747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6648..6667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgttcg taacattatg
      241 gccttaggtc acttcatctc catggggttc ttcttctgat tttctagaaa atgagatggg
      301 ggtgcagaga gcttcctcag tgacctgccc agggtcacat cagaaatgtc agagctagaa
      361 cttgaactca gattactaat cttaaattcc atgccttggg ggcatgcaag tacgatatac
      421 agaaggagtg aactcattag ggcagatgac caatgagttt aggaaagaag agtccagggc
      481 agggtacatc tacaccaccc gcccagccct gggtgagtcc agccacgttc acctcattat
      541 agttgcctct ctccagtcct accttgacgg gaagcacaag cagaaactgg gacaggagcc
      601 ccaggagacc aaatcttcat ggtccctctg ggaggatggg tggggagagc tgtggcagag
      661 gcctcaggag gggccctgct gctcagtggt gacagatagg ggtgagaaag cagacagagt
      721 cattccgtca gcattctggg tctgtttggt acttcttctc acgctaaggt ggcggtgtga
      781 tatgcacaat ggctaaaaag cagggagagc tggaaagaaa caaggacaga gacagaggcc
      841 aagtcaacca gaccaattcc cagaggaagc aaagaaacca ttacagagac tacaaggggg
      901 aagggaagga gagatgaatt agcttcccct gtaaacctta gaacccagct gttgccaggg
      961 caacggggca atacctgtct cttcagagga gatgaagttg ccagggtaac tacatcctgt
     1021 ctttctcaag gaccatccca gaatgtggca cccactagcc gttaccatag caactgcctc
     1081 tttgccccac ttaatcccat cccgtctgtt aaaagggccc tatagttgga ggtgggggag
     1141 gtaggaagag cgatgatcac ttgtggacta agtttgttca catccccttc tccaaccccc
     1201 tcagtacatc accctgggag aacaaggtcc acttgcttct gggcccacac agtcctgcag
     1261 tattgtgtat ataaggccag ggcaacggag gagcaggttt tgaagtgaaa ggcaggcagg
     1321 tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg gttgccatgg
     1381 ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc ctgctcagaa
     1441 gccccaagct cgtcaatcaa gctggttctc catttgcact caggagcacg ggcaggcgag
     1501 tggcccctag ttctgggggc agctctagag cgatcccccg accgcgggct agcggcgcgc
     1561 cgagctcgga tcctgagaac ttcagggtga gtctatggga cccttgatgt tttctttccc
     1621 cttcttttct atggttaagt tcatgtcata ggaaggggag aagtaacagg gtacacatat
     1681 tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt cttttaatat
     1741 acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca gggcaataat
     1801 gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata atttctgggt
     1861 taaggcaata gcaatatttc tgcatataaa tatttctgca tataaattgt aactgatgta
     1921 agaggtttca tattgctaat agcagctaca atccagctac cattctgctt ttattttatg
     1981 gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa tcatgttcat
     2041 acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc tggcccatca
     2101 ctttggcaaa gcacgtgaat tcgaagatct ggccgcctgg gcctctagaa ctagtggatc
     2161 ccccgggctg caggaattcg atgctaccgt cgccaccatg gcctcctccg aggacgtcat
     2221 caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     2281 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     2341 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg
     2401 ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc
     2461 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     2521 ccaggactcc tccctgcagg acggctcctt catctacaag gtgaagttca tcggcgtgaa
     2581 cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg cctccaccga
     2641 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa
     2701 ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca
     2761 gctgcccggc tactactacg tggactccaa gctggacatc acctcccaca acgaggacta
     2821 caccatcgtg gagcagtacg agcgcgccga gggccgccac cacctgttcc tgtagacggc
     2881 caaatgaatt cccaaaccgt acgggaaacg gccctattct atagtgtcac ctaaatgcta
     2941 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3001 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3061 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3121 aggacagcaa gggggaggat tgggaagaca atagcgcggc cgctctagag catggctacg
     3181 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg
     3241 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3301 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgccagctg gcgtaatagc
     3361 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaat
     3421 tccagacgat tgagcgtcaa aatgtaggta tttccatgag cgtttttcct gttgcaatgg
     3481 ctggcggtaa tattgttctg gatattacca gcaaggccga tagtttgagt tcttctactc
     3541 aggcaagtga tgttattact aatcaaagaa gtattgcgac aacggttaat ttgcgtgatg
     3601 gacagactct tttactcggt ggcctcactg attataaaaa cacttctcag gattctggcg
     3661 taccgttcct gtctaaaatc cctttaatcg gcctcctgtt tagctcccgc tctgattcta
     3721 acgaggaaag cacgttatac gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc
     3781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3841 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3961 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4021 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4081 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4141 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4201 atattaacgt ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc
     4261 tgattatcaa ccggggtaca tatgattgac atgctagttt tacgattacc gttcatcgat
     4321 tctcttgttt gctccagact ctcaggcaat gacctgatag cctttgtaga gacctctcaa
     4381 aaatagctac cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg
     4441 gtgatttgac tgtctccggc ctttctcacc cgtttgaatc tttacctaca cattactcag
     4501 gcattgcatt taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg
     4561 cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat
     4621 gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg
     4681 atgttggaat tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4741 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     4801 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4861 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4921 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4981 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5041 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5101 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5161 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5221 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5281 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5341 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5401 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5461 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5521 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5581 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5641 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5701 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5761 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5821 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5881 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5941 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6001 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6301 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6781 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6841 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6901 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6961 cgttggccga ttcattaat
//
