LOCUS       sequence-104953-        7066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses an inducible E2F2 mutant that lacks the Rb binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Vernier M, Bourdeau V, Gaumont-Leclerc MF, Moiseeva O, Begin V, Saad
            F, Mes-Masson AM, Ferbeyre G
  TITLE     Regulation of E2Fs and senescence by PML nuclear bodies.
  JOURNAL   Genes Dev. 2011 Jan 1;25(1):41-50. doi: 10.1101/gad.1975111.
  PUBMED    21205865
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-01-1- 1"; volume: "25"; issue:
            "1"; pages: "41-50"
COMMENT     Sequence Label: pBabe-ER-E2F2-dRb
FEATURES             Location/Qualifiers
     source          1..7066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1280..1302
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1318..1334
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1378..1404
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3305..3325)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3310..3639
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3490..3625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3649..4248
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(3649..3668)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4145..4165
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4347..4940
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5141..5158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5312..5900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5392..5411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6078..6869,6870..6938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6701..6720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6939..7043)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctcgttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatca tcgaattcac catggcatac
     1381 ccatacgatg ttccagatta cgctagcgga tctcgaaatg aaatgggtgc ttcaggagac
     1441 atgagggctg ccaacctttg gccaagccct cttgtgatta agcacactaa gaagaatagc
     1501 cctgccttgt ccttgacagc tgaccagatg gtcagtgcct tgttggatgc tgaaccgccc
     1561 atgatctatt ctgaatatga tccttctaga cccttcagtg aagcctcaat gatgggctta
     1621 ttgaccaacc tagcagatag ggagctggtt catatgatca actgggcaaa gagagtgcca
     1681 ggctttgggg acttgaatct ccatgatcag gtccaccttc tcgagtgtgc ctggctggag
     1741 attctgatga ttggtctcgt ctggcgctcc atggaacacc cggggaagct cctgtttgct
     1801 cctaacttgc tcctggacag gaatcaaggt aaatgtgtgg aaggcatggt ggagatcttt
     1861 gacatgttgc ttgctacgtc aagtcggttc cgcatgatga acctgcaggg tgaagagttt
     1921 gtgtgcctca aatccatcat tttgcttaat tccggagtgt acacgtttct gtccagcacc
     1981 ttgaagtctc tggaagagaa ggaccacatc caccgtgtcc tggacaagat cacagacact
     2041 ttgatccacc tgatggccaa agctggcctg actctgcagc agcagcatcg ccgcctagct
     2101 cagctccttc tcattctttc ccatatccgg catatgagta acaaacgcat ggagcatctc
     2161 tacaacatga aatgcaagaa cgtggtaccc ctctatgacc tgctcctgga gatgttggat
     2221 gcccaccgcc ttcatgcccc agccagtcgc atgggagtgc ccccagagga gcccagccag
     2281 acccagctgg ccaccaccag ctccacttca gcacattcct tacaaaccta ctacataccc
     2341 ccggaagcag agggcttccc caacacgatc ggatccatgc tgcaagggcc ccgggccttg
     2401 gcttcggccg ctgggcagac cccgaaggtg gtgcccgcga tgagccccac agagctgtgg
     2461 ccatccggcc tcagcagccc ccagctctgc ccagctactg ctacctacta cacaccgctg
     2521 tacccgcaga cggcgcctcc cgcagcggcg ccaggcacct gcctcgacgc cactccccac
     2581 ggacccgagg gccaagttgt gcgatgcctg ccggcaggcc ggctgccggc caaaaggaag
     2641 ctggatctgg aggggattgg gaggcccgtc gtccctgagt tcccaacccc caaggggaag
     2701 tgcatcagag tggatggcct ccccagcccc aaaaccccca aatcccccgg ggagaagact
     2761 cggtatgaca cttcgctggg gctgctcacc aagaagttca tttacctcct gagcgagtca
     2821 gaggatgggg tcctggacct gaactgggcc gctgaggtgc tggacgtgca gaagcggcgc
     2881 atctatgaca tcaccaacgt gctggaaggc atccagctca tccgcaagaa ggccaagaac
     2941 aacatccagt gggtaggcag gggaatgttt gaagacccca ccagacctgg gaagcagcaa
     3001 cagctggggc aggagctgaa ggagctgatg aacacggagc aggccttgga ccagctcatc
     3061 cagagctgct ctctgagctt caagcacctg actgaggaca aggccaacaa gaggctggcc
     3121 tatgtgactt accaggatat ccgtgctgtt ggcaacttta aggagcagac agtgattgcc
     3181 gtcaaggccc ctccgcagac gagactggaa gtgcccgaca ggactgagga caacctgcag
     3241 atatatctca agagcaccca agggcccatc gaagtctact gaggatagtt aacgtcgacc
     3301 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3361 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3421 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3481 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3541 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3601 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac
     3661 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3721 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3781 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3841 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     3901 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3961 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4021 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4081 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4141 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgagtgc
     4201 ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     4261 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataaa ataaaagatt
     4321 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     4381 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc
     4441 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     4501 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     4561 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     4621 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     4681 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     4741 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     4801 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     4861 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     4921 gtcagcgggg gtctttcatt tccgacttgt ggtctcgctg ccttgggagg gtctcctctg
     4981 agtgattgac tacccgtcag cgggggtctt cacatgcagc atgtatcaaa attaatttgg
     5041 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
     5101 gcggggagag gcggtttgcg tattggcgct cttccgcttc ctcgctcact gactcgctgc
     5161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     5761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5821 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5881 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5941 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6001 agatcctttt aaattaaaaa tgaagtttgc ggccgcaaat caatctaaag tatatatgag
     6061 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6121 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6181 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6241 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6301 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6361 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6421 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6481 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6541 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6601 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6661 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     6721 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6781 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6841 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6901 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6961 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7021 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
