LOCUS       sequence-10501-e        8403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 8403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-fTCAP-GFP
FEATURES             Location/Qualifiers
     source          1..8403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     intron          2952..3427
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     3509..3525
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3509..3525
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      3558..3567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3564..3566,3567..3569,3570..4280)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3564..3566,3567..3569,3570..4280)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3609..3630)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3870..3889)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4217..4238
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4341..4359)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4342..4359)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4379..4396)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4385..4496
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5187..5700
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5274..5293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5484..5505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6181..6200)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6300..6322
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6360..6378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6446..6550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6551..6619,6620..7411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6769..6788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7582..8170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8071..8090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtggc cggccatgac
      241 agtgaaagag aggatggaat tttgagtgat tgatcctaag acccacctgc aataataaaa
      301 gacacaaggc agggggcagc agccattagg cctggagacc caagacacaa atgcgtagct
      361 tttctttccc cttatgcaga tttcaaagtt ttactgtgtc aatcttttct ttattcttct
      421 gtctgcagag cttgatgcta attctgcttt gtgctgaaac atgaaagggt catcaggcga
      481 atgtgacaga aatattaagt gacagttctc tccggttgag tcctttccag ttttggccct
      541 gttcactgtt cagctgttgt gagccttcag gggagcagtg ttcttgatat tctgtcctgt
      601 gattatcagc acttgcttcc tttgatcaca tgacccagcc cagaactctg atgtcagaat
      661 atttttttta tgtttcacat gattgggttt gtgtaattgc tggatttaac ttacattcag
      721 catctcagat gctttgggaa tagcagatgg cgatattacg tacggtgaac ttaaaaaccg
      781 ggtttgtacg tgactgacgt ttcttccagg atgtctgtgt gtgtttaatc taacagtgaa
      841 ctgaaatcaa gggtaatttc tcaaactcac atggtcgatt gtgtgtgcat gtgtgtgtgt
      901 tgtggtgtcc agttcaggat gaaataaagt gatgtgaagc atgttacggg cagcgtgtca
      961 ttagaaagtc aagctgcttc tttctctttt caagcgctca cgtatcctgt tggctgtcaa
     1021 ccatgggctg aattactgtc tgatctgcag tcatgccaga aaatcccggc ctctctggtg
     1081 tctgactgtg ccgctgggac cagtcaggga ggaagatgaa tggtgatgat agtgttttgg
     1141 tgctcagctg ctatttcttg ttgttattgt tatataagag agacattgga gggggcgagg
     1201 gtggcacatg tttcaacacg gcaccagagt tttagcacac acacacacac acacacacac
     1261 acacacattg ctggtaaccc ttgagagtgc agtgctgaag tagatgtgca ggaagttaac
     1321 tttctcgtca agcacgcaac gtcacgctga ccagccgacc ttctcgttgg tgactcaagt
     1381 cgttcacttt catgatattt ggctctattt gccaccttcc gacaggcccg ggcttcatcc
     1441 agcgatcgcc cttctccctc tctccctccc tctcccagtc tctctgctgg cgacagctca
     1501 tttaagtgtc acttcccagc agctgtggca gccttgttat gcaacgccat gttcttccgc
     1561 aatgtcggcc tgttacaggt ggagggacgg tggggggatg acgtgactta cctcatcggt
     1621 gttcctccaa atggactctt tacaccctcg tctagcacga gtcttaagaa cgacatgacg
     1681 aagttaaaac aataaatcat ccacccccgt tagtcactgc tacgtcaggt tcgttctgga
     1741 gtgacatcat ctcaaaccaa cttactgggt ttcagaaatg gcattttatg gttgtcatgg
     1801 tgtattgaat gaccccatga gtgtattctg gctctgggtt ctttcaatga caccaagcag
     1861 tggtatgcaa ccctactgag aagaaattta aatacagtta aaagccattg gaaaataaca
     1921 tgtaactgat gtattagaag tagatgttat tggtcagtgg agaagagtgg cggttattta
     1981 agagctctag ttgtacggaa ttgctttgat cattttttaa gagaccgggc ttttgccaca
     2041 tgtgtgaaat cagaaattta atgtcgaatg tggatcaatg cgccaccgag tggcaccggt
     2101 ttcgcatctt tacattctgt aacctgagtc aagtagacat ggaatgaccc gctgtcaccg
     2161 atgtactgaa caacggtgct gtcatggcat atttgactct cagcagttga atggttcccc
     2221 gctgttttat ttctttagaa tttcagaggt tttgtcacat ttatcttaac ctcctctgtg
     2281 tatttgagac cactgcgtgg ttatcttccc acattagcat gttgtcctgc tcccccatgt
     2341 acattattgc ctaaacttta ttaagactct ctctaatcaa ctaaacctgt tgtttgtgtc
     2401 ggtctgcatg tttatctaat ctaagtctta tttaagtcca catgtttcca ccgcgcccga
     2461 gtgatcctct ctgacgtgtc aagtgtacag ctgagtgagg gacgaggtga ctggccacat
     2521 aattccatag aatttgagca tcgggggatt ttcaagccta ttacccttaa cccctcgact
     2581 gagtggcttc cagttctcaa caggaatgag acacagagca ctcggtgtcg gatgtctctg
     2641 cacagtgatt gtcgagtgac aacatgactc agaaatagaa acagaagatc tataaaagcg
     2701 gtgggtggat cagggctcgg acacagacgg ttcagaggag tgaatccggg agccaccccg
     2761 agcatcacag ttgcctcatc acaggcatca cttgaccccc cgagacgcat ctttctgaga
     2821 cgaatttccc tgcagcagac ataatgccag tctgttcaat cctggagaag tgtaatgatg
     2881 aggtgatggg cgctgagctg acctgcagtt tggctagcgg cgcgccgagc tcggatcctg
     2941 agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct tttctatggt
     3001 taagttcatg tcataggaag gggagaagta acagggtaca catattgacc aaatcagggt
     3061 aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc
     3121 ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat
     3181 gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg caatagcaat
     3241 atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg
     3301 ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg
     3361 gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct
     3421 cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg gcaaagcacg
     3481 tgaattcgaa gatctggccg cctgggcctc tagaactagt ggatcccccg ggctgcagga
     3541 attcgatgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     3601 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     3661 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3721 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3781 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3841 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3901 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3961 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     4021 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     4081 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4141 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     4201 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4261 gcatggacga gctgtacaag tccggactca gatcctacta gtaggatctc gagtttaaac
     4321 cgtacgggaa acggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc
     4381 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4441 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4501 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4561 gattgggaag acaatagcgc ggccgctcta gagcatggct acgtagataa gtagcatggc
     4621 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4681 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4741 gcggcctcag tgagcgagcg agcgcgccag ctggcgtaat agcgaagagg cccgcaccga
     4801 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg aattccagac gattgagcgt
     4861 caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt
     4921 ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt
     4981 actaatcaaa gaagtattgc gacaacggtt aatttgcgtg atggacagac tcttttactc
     5041 ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt cctgtctaaa
     5101 atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga aagcacgtta
     5161 tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     5221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5581 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5641 ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat caaccggggt
     5701 acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg tttgctccag
     5761 actctcaggc aatgacctga tagcctttgt agagacctct caaaaatagc taccctctcc
     5821 ggcatgaatt tatcagctag aacggttgaa tatcatattg atggtgattt gactgtctcc
     5881 ggcctttctc acccgtttga atctttacct acacattact caggcattgc atttaaaata
     5941 tatgagggtt ctaaaaattt ttatccttgc gttgaaataa aggcttctcc cgcaaaagta
     6001 ttacagggtc ataatgtttt tggtacaacc gatttagctt tatgctctga ggctttattg
     6061 cttaattttg ctaattcttt gccttgcctg tatgatttat tggatgttgg aattcctgat
     6121 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     6181 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     6241 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6301 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     6361 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6421 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6481 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6541 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6601 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6661 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6721 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6781 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6841 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6901 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6961 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7021 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7081 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7141 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7201 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7261 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7321 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7381 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7441 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7501 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7561 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7621 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7681 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7741 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7801 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7861 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7921 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7981 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8041 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8101 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8161 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8221 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8281 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8341 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8401 atg
//
