LOCUS       sequence-10502-e        8291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 8291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-fCAMK-GFP
FEATURES             Location/Qualifiers
     source          1..8291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     intron          2840..3315
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     3397..3413
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3397..3413
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      3446..3455
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3452..3454,3455..3457,3458..4168)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3452..3454,3455..3457,3458..4168)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3497..3518)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3758..3777)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4105..4126
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4229..4247)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4230..4247)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4267..4284)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4273..4384
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5075..5588
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5162..5181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5372..5393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6069..6088)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6188..6210
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6248..6266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6334..6438
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6439..6507,6508..7299)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6657..6676)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7470..8058
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7959..7978
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtagt ctggagacca
      241 agttcaggac tccggagacc cagtccaggc ctctggggtt ccagtccagg acaataaaga
      301 agcagttcag gattctaaag aaccaatcca ggatgcaggc tctcagccat gatgtaattt
      361 tgcaggaaat agattttctg ctctcttctc atcctacatg aactctgcaa aatacagaat
      421 tggtttctta tcatcttcac tgtgaccagg gagtggaatc attgaggcat gaacacaggt
      481 cttaattctg aatgtgtttg tgtcacagac tattagcttt actcagctca tactttcaca
      541 gagattaatc atgaattatg aatcatgaaa tgatcttgat gaataaatct tcacactgga
      601 acatcttcat ttccgtgctt ctgttgtggg tgaaagatca ctgctccctt ctggctggat
      661 aaaaaaaaaa gacatcaaga aatatgaaga tatttaaatt ataaatggtg gtagtgacga
      721 ctggtggtgc agttcaaatc accaggtaat caatcgccga tttgaaatat gctgtacccg
      781 cccacagccg ccaggggacg ctagtcaaca acaggacatt taaatgtcag tgaaggaatt
      841 tatcctgtaa aatcttgcga tgtgccaata ttaatgccaa aaaaaactga tataacactg
      901 gctggagaat gaataatcat cagatgagtt tacataagcg ataatggtgt ttggtctgtg
      961 tttgaaattg ctggttttgt tattctctgc aatattgctg aattgtgaac tgaaaggttt
     1021 caaccagaca cataagctga atcgaatcga tgagttttaa tgaggaaacg attgcttgtg
     1081 tcaaaagagg catctaatgg taaaattgtt aagggcagct ttaagcaatt gatggaaaca
     1141 gctggtgctg agctcacgag gtcacatttg catctctatc taagtgatat tgcactaatg
     1201 gaagaatcca tttaaaagtt gggatcccct ccatttaata gaccacctgc gtctgacctt
     1261 ctgtcaccgt gtcatctgct acagtgggaa acgatcataa agcagcatgt gacatatgtt
     1321 tcctcattag tccagtgctc ccagtgcccc cagtgctgcc ttcagtgagc cgtctgttta
     1381 attccacttc tgtcaaacaa acatggagtt tgaactctta acgatggtgt tttgtttgag
     1441 tccagcagtc atgcttcaag caaccgtgag caggtctttc taatatgaat tctccttacc
     1501 agccaattcc tgctcctcct cctcttcctc ctcctcttct gctgggacag cgaggaggct
     1561 ccaaagaggg cagccatgca gaactgatga gtgacagaaa gagaggagca gaattgagag
     1621 aggctgctcc cggcagacac aaacaaaccc caaaatagac taaagaacaa gaaggctcag
     1681 tccctctgac ctcaggttga aaagacacta cagacagctg caatatcacc ctcgctccgc
     1741 tcaccggttc agttacacac acccgattta acagtttgtt ctttttcttc acatgcagca
     1801 aaccaaagtc cgctcagatg gtgaatgggt tgtaaatttg ctattgtaac cacgaacaac
     1861 agagcaggtg gggtaaatct agcaccagtt gtcaggcctt ctctgtgagt ctgttcattt
     1921 cgtctgtcct aaaattcctt atgctggttt tctttattcc ttcaccattg gtgggagcgt
     1981 cccagttccc cccccggttc cattactgca cgtagtatcg gaccattcag tgtccttgga
     2041 tacgctggac gcagactcac acatgaaatt tcaggtaaac gccgcctgtt gtgtgttgtt
     2101 cgcctcccac aggtcacctc ctctctggaa aatcttactg cgagactttg tagtactggt
     2161 gagtagcaag agcagcttta tcttgtagta gcactgaagt tagcacaagt ataagcagtc
     2221 agatcggaag taattttgaa tgtgatggga gctgaggagt ggcgttccgg acgtgttctc
     2281 tatggttttt ctatgtacaa taatatttat agtactgcag ttttatttcc cgaataatct
     2341 gatgctgttt gcagttactt tccagcacac acagagggga tgcagttacc atggtgaccc
     2401 tgtgcatgtg agtgtacatg catgcgtgtg tgtgtctgtc ccggtctgtg attggctgat
     2461 gggccagggt gggtgggcag gtggagcgtc tgaaagcatc tttctcccac agacccggac
     2521 agagagacag agagacacag atctcctaac agctctctcc ttcttccctt catcttcctc
     2581 cttttcctcc tcttcctcat cctcctcctc ctcttcctct cctcgggact gaaactatct
     2641 ctccatcact cttctactct ctctattttc tctcctccat ccttctggca gacagaacgg
     2701 cagctctaac tcttggcctc cttcgtctcc ggcatcatca tcttcagtgg aagacggaca
     2761 gggaccccct tgaccctcga cctacagggt caccagggat gctagcggcg cgccgagctc
     2821 ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt ccccttcttt
     2881 tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca tattgaccaa
     2941 atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa tatacttttt
     3001 tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat aatgatacaa
     3061 tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca
     3121 atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat gtaagaggtt
     3181 tcatattgct aatagcagct acaatccagc taccattctg cttttatttt atggttggga
     3241 taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt catacctctt
     3301 atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca tcactttggc
     3361 aaagcacgtg aattcgaaga tctggccgcc tgggcctcta gaactagtgg atcccccggg
     3421 ctgcaggaat tcgatgctac cggtcgccac catggtgagc aagggcgagg agctgttcac
     3481 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     3541 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     3601 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     3661 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     3721 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     3781 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     3841 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     3901 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3961 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4021 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4081 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4141 cactctcggc atggacgagc tgtacaagtc cggactcaga tcctactagt aggatctcga
     4201 gtttaaaccg tacgggaaac ggccctattc tatagtgtca cctaaatgct agagctcgct
     4261 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4321 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4381 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4441 agggggagga ttgggaagac aatagcgcgg ccgctctaga gcatggctac gtagataagt
     4501 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     4561 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     4621 ttgcccgggc ggcctcagtg agcgagcgag cgcgccagct ggcgtaatag cgaagaggcc
     4681 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa ttccagacga
     4741 ttgagcgtca aaatgtaggt atttccatga gcgtttttcc tgttgcaatg gctggcggta
     4801 atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact caggcaagtg
     4861 atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat ggacagactc
     4921 ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc gtaccgttcc
     4981 tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct aacgaggaaa
     5041 gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     5101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5221 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5281 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5341 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5401 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5461 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5521 tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt ctgattatca
     5581 accggggtac atatgattga catgctagtt ttacgattac cgttcatcga ttctcttgtt
     5641 tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca aaaatagcta
     5701 ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat ggtgatttga
     5761 ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca ggcattgcat
     5821 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg
     5881 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg
     5941 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttggaa
     6001 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     6061 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6121 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6181 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6301 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6361 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6421 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6481 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6541 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6601 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6661 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6721 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6781 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6841 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6901 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6961 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7021 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7081 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7141 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7201 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7261 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7321 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7381 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7441 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7501 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7561 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7621 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7681 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7741 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7801 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7861 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7921 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7981 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8041 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8101 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8161 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8221 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8281 attcattaat g
//
