LOCUS       sequence-10506-e        8128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-hADM-RFP
FEATURES             Location/Qualifiers
     source          1..8128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     intron          2802..3277
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      3353..3362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3359..4036
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3489..3507)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3539..3559)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3757..3776
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3951..3974
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(4066..4084)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4067..4084)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4104..4121)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4110..4221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4912..5425
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4999..5018)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5209..5230
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5906..5925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6025..6047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6085..6103)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6171..6275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6276..6344,6345..7136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6494..6513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7307..7895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7796..7815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacagagtga aaaggcagct aagccagcag
      241 tgcgcggccc agacagacca ctctgggcaa ggtccgcgcg acccactgcc cagccgcctt
      301 tgcaccggag ctcagagcca tatggcagag taggaaggaa gcttaaaggt ctgctgggga
      361 ccggctctaa gatggggact cgagagatgg gacatgactc gcccaccttc acacagcgaa
      421 ttgccgggag gtgaggacta gaagcctgtt tggctagtgg tctcgcacct ttggcctctc
      481 cgtcgagtcg ctgggcttca ggacattcga ttagaggagt ggttagtagg accccagaaa
      541 cccacaagca cgaagcagga agccccgtgg cttagccttt ccttcccagt ttggccccca
      601 ggagagaagt aagaaagaga agaagctgtg atgaaagagc acaaacgggt gacaaacgtg
      661 tctagcgtga ttcatcatga acaggcacaa attctttggg cgggggctag gactctcctt
      721 tgccccttga gaagttggtg accccagcct agaggaatcc acgccgcgcc ccagtcggcc
      781 gtgctagcgt gtcgggccga gtagggactc tgctgcttcc tacctgcagg gtgacttccc
      841 ctctctgcag atacctcccc tctctgagct tgaattttct acctgctgaa atgggaaaag
      901 gaatgttacc ttccttgcct gactcaaggg tggctgtgaa gctcaaagta gactgggatg
      961 tggccgtgct tggtaaactg taaaatgatt agcatacgtg aagcgttagt gtgctccctg
     1021 gcagtcagtc ctcacgttta cgatggatta atgaaggcag ccaggcacaa tctcaggtta
     1081 tgaccttata aggcataata ggattacaag gaggcaaaat gaaacagtat atttcctcct
     1141 aattttggtt attcctatgt ttccaaatag aaatgaaaat tctgaaattt cagataattc
     1201 ccccctccaa atcccaattc tcaatttagc ttttctgagc cttgtcccta ccgtgaaatc
     1261 ttactagctc accccgaaac gccggggtct tggtcctctg ggggactagt gagtcctgct
     1321 cctctcggct ttgctccgtc cctgccccgg cccctccgcg ggagtctggg gtcgcggccc
     1381 ggtcaggcgc caagccggca ggggggcgcg gtccacttga ggccacagct ccccaggtcc
     1441 agggctcggg ccatccgcgc tgtcccttcc gcgggctctt gctgttcttc gccaggaggc
     1501 tttgcactcc gggccgcatg ggtcctggat aaggacctca agaggttgta gtggcgcccc
     1561 ttcgctccgg cgtactgtct gaaccctgtg cccaaagagg gctgtggcag ttcctgcacc
     1621 aaccgcctgg agcccatacc taagccctct gggcacgagg gacctcctct cccccgctcc
     1681 cccctccccc catcccaact ccagccccaa aggaagcaat gcgcgcgtcc gagagcagga
     1741 gcgcgcgtgg ctgaggaaag aaagggaagg caaccgggca gcccaggccc cgccccgccg
     1801 ctcccccacc cgtgcgctta taaagcacag gaaccagagc tggccactca gtggtttctt
     1861 ggtgacactg gatagaacag ctcaagcctt gccacttcgg gcttctcact gcagctgggc
     1921 ttggacttcg gagttttgcc attgccagtg ggacgtctga gactttctcc ttcaagtact
     1981 tggcagatca ctctcttagc aggtaggtgc cgcagaccct gcgggttaag aggtggggtg
     2041 gggggcagtg cttgccaagg ccctaacctg ggagcgctgg gtgaggggaa caacccactt
     2101 tggagggttc tctgagagat agatacaccc catatcctgg gcccagctcg tgcacacagc
     2161 tggaggtcca gagacccagt cccctctgct ccgtcagcca agttccaaga agttgagcag
     2221 agaccctctg ggagcctggc ggggtgcagc ggcctcccct gcggggcctg tcacccggcc
     2281 ggcgcgtgca aacgcctctg gcgcctctct gcgcggaggg agataagcgt ctgagccagg
     2341 gaaagcgcgg gctaaacccg cctcgccggg gcccctgccc gccctccgtg ccccgcccgg
     2401 gcggtgcagc tggcccgggt gctcacgctc gactctcttt cttcttttcc agggtctgcg
     2461 cttcgcagcc gggatgaagc tggtttccgt cgccctgatg tacctgggtt cgctcgcctt
     2521 cctaggcgct gacaccgctc ggttggatgt cgcgtcggag tttcgaaaga agtgagtccg
     2581 ggcagcgcct tcccccttgc tggtacctgg caggcaaggg gaactgaccg ttggtcccga
     2641 aggtctagaa gtgaatggga gcagggacag gcctgggcgt cacctgaacg cacgcgaatc
     2701 gggtctgctt gtgttttcca ggtggaataa gtgggctctg agtcgtggga agagggaact
     2761 gcggatgtgg cgcgccgagc tcggatcctg agaacttcag ggtgagtcta tgggaccctt
     2821 gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta
     2881 acagggtaca catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct
     2941 ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc
     3001 tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag
     3061 tgataatttc tgggttaagg caatagcaat atttctgcat ataaatattt ctgcatataa
     3121 attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc
     3181 tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt
     3241 gctaatcatg ttcatacctc ttatcttcct cccacagctc ctgggcaacg tgctggtctg
     3301 tgtgctggcc catcactttg gcaaagcacg tgggccgcct cggccaccgg tcgccaccat
     3361 ggcctcctcc gaggacgtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
     3421 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3481 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     3541 gtccccccag ttccagtacg gctccaaggt gtacgtgaag caccccgccg acatccccga
     3601 ctacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3661 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggctcct tcatctacaa
     3721 ggtgaagttc atcggcgtga acttcccctc cgacggcccc gtaatgcaga agaagactat
     3781 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     3841 ccacaaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agtccatcta
     3901 catggccaag aagcccgtgc agctgcccgg ctactactac gtggactcca agctggacat
     3961 cacctcccac aacgaggact acaccatcgt ggagcagtac gagcgcgccg agggccgcca
     4021 ccacctgttc ctgtagagtt taaaccgtac gggaaacggc cctattctat agtgtcacct
     4081 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4141 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4201 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4261 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcgcggccg ctctagagca
     4321 tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat
     4381 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     4441 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gccagctggc
     4501 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4561 aatggaattc cagacgattg agcgtcaaaa tgtaggtatt tccatgagcg tttttcctgt
     4621 tgcaatggct ggcggtaata ttgttctgga tattaccagc aaggccgata gtttgagttc
     4681 ttctactcag gcaagtgatg ttattactaa tcaaagaagt attgcgacaa cggttaattt
     4741 gcgtgatgga cagactcttt tactcggtgg cctcactgat tataaaaaca cttctcagga
     4801 ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta gctcccgctc
     4861 tgattctaac gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct
     4921 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4981 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5041 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5101 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5161 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5221 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5281 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5341 ttaacaaaat attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
     5401 ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt
     5461 tcatcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagaga
     5521 cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca
     5581 tattgatggt gatttgactg tctccggcct ttctcacccg tttgaatctt tacctacaca
     5641 ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga
     5701 aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt
     5761 agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga
     5821 tttattggat gttggaattc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5881 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5941 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6001 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6061 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6121 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6241 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6301 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6361 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6421 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6481 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6541 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6601 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6661 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6721 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6781 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6841 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6901 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6961 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7021 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7081 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7141 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7201 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7261 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7321 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7381 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7441 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7501 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7561 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7621 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7681 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7741 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7801 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7861 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7921 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7981 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8041 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8101 tccccgcgcg ttggccgatt cattaatg
//
