LOCUS       sequence-10508-e        8386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-hRREB1-RFP
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     promoter        2963..3001
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          3060..3535
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      3611..3620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3617..4294
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3747..3765)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3797..3817)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4015..4034
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4209..4232
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(4324..4342)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4325..4342)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4362..4379)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4368..4479
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5170..5683
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5257..5276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5467..5488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6164..6183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6283..6305
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6343..6361)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6429..6533
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6534..6602,6603..7394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6752..6771)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7565..8153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8054..8073
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttaca atggcttaca cctgtaatcc cagcaatttg
      241 ggaggccaag gtgggagaat cacttgagcc ctagagcctg agaccagcct gggcaacata
      301 gcaagacctc ctctctacta aaacttttaa aattaactag atgtggtgac atgcactgta
      361 gtcccaccta cttgggaggc taaaggtgga ggatcacttg agaccaggaa ttcaaagctg
      421 cagtgagttg tgatcccacc actgaactcc agcccgggca acagagtgat gctctgtctc
      481 caaaaaaaaa aaaaaattaa aaaaagttgt agtaaaactg ttttcaatct tttttgttgc
      541 cagtattttc tagatggcat tatacaggtg gcaatttgtc atgaaaattc atatagcatt
      601 ggagatttgc taaattatgc ataaaattcc aattttgata tttttcctat tcaaaactta
      661 tccctgaatg gggagaccag gaaaggctag ctcctgaatc agagacaaag tgagtaaata
      721 aagtagcatt ccccacaggg cccttcctct gccctcattg gaacacatta caacttagaa
      781 acacaagaca cctattctat gatggcaatg tgaactatta ttcctgtcta gagacctgcc
      841 catctcctcc acgactggcc tgtcttcaaa aaagcatttg ataatacatt tgggggcggc
      901 ggggttgggg gaggctcaga aaaatgcaac cgctaaaatt tctctggatg gagttttctt
      961 cagtaaacag tagaccctgg gaaacataac aggttccgaa aacttaattt tgatgactga
     1021 aagtcagaga aacagccagc tgaatcagct ccttcttggg cagtggttta gcctacttat
     1081 ctcatatcag acctaagggt ccagctgctt ttccttggtt ctaaagagtt ctattctggc
     1141 caggacccat cccgagacag acacttctgt ctgcagccct gaaaactaga ggtcaatgga
     1201 agggctcaaa ttaaaggccc acagttgggc ttacaactct ccatggtgtt caagccttct
     1261 cagaggagct ggtgtggtgg agccagggtc aaaggtcata gtaaatagaa agtagccagc
     1321 tttctgcctg cataacatca tcttgcctaa ccagactcaa acgcttaatt ctactgtagt
     1381 attctttgga cattcttctt tagaactacg ggggcggggg ggggggggcg tgtttatttt
     1441 caacaaccac aatcatcctt atctttctga agatgtttcc aaaggctgag aacagacccc
     1501 tttcagaacc tcggtaggag aagtattaac agttggaaac atgtcagctg ctttatggag
     1561 gaagcacagc ccaggcctgg tttctctggg ctccgtgata acttggggca aggcagccag
     1621 tgcgaagcgt ggtggcagga gctggagaga tggagagcgg ctcggagaag ggcccctgct
     1681 tttcagagtt gaaacaggag ccctctgaga gaaagagata ctgcgtgtgg cttctctgaa
     1741 gccttggagg gcgacgtgga tgacacagca tcagccattc catcgcgggg tggggacaca
     1801 gtggcggcag gaaccgtcct cagcccctgg cggccacggc agttccccgc tgaagcatcc
     1861 tttgtggggt gtgggtaagg gagggggacc aaagtcgggt cggagttgag tttgttagac
     1921 ggcgagcaca ctcagggcgg cagcccgagt aagaaggatg gccccgggag agaaacggct
     1981 cgaagctagg ggcggggtga agcgcctgct ccttaatctc ctgccccatc atccactcgc
     2041 cccacctgga tgcagagcga ggaccgcgtg gaaaggaaac ggcgcgggcg gggctccccc
     2101 tcgcagatgc ctcggagacc cagaggggcg gcgtcaggcc agggccccga gccctacgcc
     2161 ccccatcccc ccaactccag cgcctctgag gtgcccctct cgcaattccg aggcatcacc
     2221 gtgtctcccc cgcctccgtt aaaatagtga aactgcggag tgaagtgatg gaaagccggg
     2281 ggcggggagt cgcccgcggc gaggggctgc aggggagggg gcgcggtggc cgaataaggt
     2341 ggcccgcgag caacgggacg attgggatcg cagccagtcg cggcctcccg gacccttccc
     2401 ctcgggcagc ccggccctcc catggcccgg ggcgccttgg gagggcgtag ggcaaacctg
     2461 gggctgcggt ggggggtggg gaggggactc cacctccatc catcccggga tggcaactgc
     2521 ggtcaccctg ctaaagtcgg ggcgggggcg gcggtcctcc ccctcacccc ccccagtccg
     2581 agcgccgccg ccgccgccgc cgccgccgcc gcggccgctc agtaacacgt ccccaggaga
     2641 ctcgcaggag caacacgtga tgtgtctact tatcagggtg agagggggag agcgagactc
     2701 cgagcgtgcg ggcgaggaag ctgggcgggg ggccgagacg ggggccggga cgacggctgg
     2761 ggcgccgggg tcgccagctc ctcgcccacc tccctccctg tgggagaaga ggaggagggg
     2821 aagtgactgc ggagttgatg aactttgcac atccagaaac gccattttga ttccttttcc
     2881 ggaacaagtt tgcatctctc ctcggtctct ccctgaagcc caccggtccc caacgcatca
     2941 tgccagggag ccaattaatt aaggtaggcg tgtacggtgg gaggtctata taagcagagc
     3001 tggtttagtg aaccgtcaga tccgctagcg cgccgagctc ggatcctgag aacttcaggg
     3061 tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc
     3121 ataggaaggg gagaagtaac agggtacaca tattgaccaa atcagggtaa ttttgcattt
     3181 gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata
     3241 ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac
     3301 cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat
     3361 aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct
     3421 acaatccagc taccattctg cttttatttt atggttggga taaggctgga ttattctgag
     3481 tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct
     3541 gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaagcacgtg ggccgcctcg
     3601 gccaccggtc gccaccatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa
     3661 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3721 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     3781 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
     3841 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
     3901 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     3961 cggctccttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
     4021 aatgcagaag aagactatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     4081 cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc actacctggt
     4141 ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
     4201 ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga
     4261 gcgcgccgag ggccgccacc acctgttcct gtagagttta aaccgtacgg gaaacggccc
     4321 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
     4381 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4441 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4501 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4561 cgcggccgct ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     4621 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4681 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4741 gcgagcgcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4801 tgcgcagcct gaatggcgaa tggaattcca gacgattgag cgtcaaaatg taggtatttc
     4861 catgagcgtt tttcctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa
     4921 ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat
     4981 tgcgacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta
     5041 taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct
     5101 cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc
     5161 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5221 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5281 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5341 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5401 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5461 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5521 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5581 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata
     5641 caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc
     5701 tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc
     5761 tgatagcctt tgtagagacc tctcaaaaat agctaccctc tccggcatga atttatcagc
     5821 tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt ctcacccgtt
     5881 tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg gttctaaaaa
     5941 tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg gtcataatgt
     6001 ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt ttgctaattc
     6061 tttgccttgc ctgtatgatt tattggatgt tggaattcct gatgcggtat tttctcctta
     6121 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     6181 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     6241 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6301 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     6361 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6421 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6481 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6541 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6601 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6661 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6721 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6781 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6841 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6901 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6961 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7021 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7081 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7141 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7201 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7261 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7321 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7381 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7441 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7501 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7561 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7621 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7681 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     7741 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7801 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7861 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7921 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7981 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8041 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8101 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8161 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8221 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8281 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8341 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatg
//
