LOCUS       sequence-10510-e        8129 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-hNR2F2-RFP
FEATURES             Location/Qualifiers
     source          1..8129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     CDS             371..382
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     intron          2803..3278
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      3354..3363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3360..4037
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(3490..3508)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3540..3560)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3758..3777
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3952..3975
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(4067..4085)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4068..4085)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4105..4122)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4111..4222
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4913..5426
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5000..5019)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5210..5231
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5907..5926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6026..6048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6086..6104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6172..6276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6277..6345,6346..7137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6495..6514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7308..7896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7797..7816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aattggtccg tcttgactgt ccatataaaa caatttaatt
      241 agggtgcttt cttgctttgg agagcaaaga aaaaaaaaaa aaaaaccctg cccagtgctt
      301 attgtattta gcaccaatac aggcagaaaa tgtagcacaa gtttttggtt gaaagagcac
      361 ctctgtgccc attgaagggc gttgatccga aaagacagaa aggacaagta aaatcgattt
      421 gaagcgaccc tctctgtccc tttcacacca ttcagaaact ccattccttc taagacaaag
      481 accagcgcct tgggaattct cagggaaaaa gaggactcta gtcccacaag cctagggcac
      541 ttttttaaaa aaaaatcctc aatatttcac ttaaaaccca cacttgatgc tgtgcccccc
      601 aagtatgggt tccacagatg ccccctccaa agtgccaagc tgcaaggctg acaaaccccc
      661 tttcccctgc ctgtttggcc ttgtagccag gccaggacag ccgtttaggg ttagtgcagc
      721 cctcaccttt gacctcctag atccgagtgg ctgccagcac tacaactttg gccaaagtta
      781 aaagaaaacc acaacaaaaa attttttctg ctcccaaacc cagggaaacc agttctggtt
      841 cagccgcctg agccacctta cccaatagca acctccatcc ctaacttttt tttcttcttc
      901 ttttgcaatg aactgcctga gccatctctg aaggagggaa ttgaagttgt cagggctcca
      961 cggctgggtt gtgggtgggg gtgagtttct tggtctaact ggtcctccat taggaacccc
     1021 cgaatgccag gttatcccac tggtgcccga gctggggaag gcagtcatct atcagaactg
     1081 gcaggaaagg gagaagggag aggaatgaga tgggcaggga ggggagagaa aaggccaggc
     1141 tgtgatcatc ttcacctgcc ctgagtccag ccttcctgtt caactattcc tcgaccttcg
     1201 gctggggatg aagggggcgg gggcgaaagg gagggggcac ccccgagtta cacaagtggc
     1261 ctccttctac taagagaatg agttcacctc ctccccagag gagcctcacc atccatccac
     1321 tcagtataac tgatgagaca aaccgcaagg aaaaacttct gtagcccata tttatggtat
     1381 taatgaatag ttaaaaaaga tttacacttc ttatgatcaa tattctgagt tattttgctc
     1441 aaagtatata attacagtgt cttcagagtt ttcatcattg tttgaggtgg caaaaccatt
     1501 tgtaattttc attagccagt aaacatgtaa ttaacattca gcactgtgtt taacttaatt
     1561 cctgtttaag acaaggacaa gaagcagccg aagagcgtta gttccatttg tcaagtctga
     1621 catacatctt accataacag tttcctaaaa taaaagatcc gtgtttcccc attattcttc
     1681 tcttaaaaca aaacaaaaca aaaatccatt agttaagaca gtattaaact gtgagaggca
     1741 gcatagttta tattcaattt tcactagtta ccgtattctt gcttccttta ttcgttttgg
     1801 ggagtagaga aagggagggg gtgaatgaag cattcattat ctgaaggcat ggaatgggga
     1861 acccctcccc ccaaaagaaa tagatttcct cctcaggaaa gttctagaat aaggaccgga
     1921 aaggggggag gcagaaatcc acaggggaga gggggaaaaa tcacttgggg agacttgagt
     1981 taaaagtaga aaatgaggat tccaaaggat tcctaggaaa aatgttgccc tcttgtctca
     2041 agttctatct ttgatccagg acgagggaag cctgtctgcc agtctgcgag gctgatgctt
     2101 tttccccctc ctcttccacc tctgtttttt cccaacttgc tttaggatta gcctccatct
     2161 ctctcgaccc caaacagtgg aatttggctg tgccttgggt cgtcttgctt tgcaatatcg
     2221 cctatagttg ttggcggttt tctgctgagg ctgagccgtt tgctccagcc tccgactaaa
     2281 ctcattaagt tgggagattt ttttcaattg gacgggtgtt tttaaagtct cgtttttcca
     2341 gccccaaaca aggtgtaaca acgcactctt ccttctaagg aatgagatga gagacaagga
     2401 tcactccaga catctcctac ctacggtttg gggttttttt tcttaaaggc gaggcttgca
     2461 ttcctcagca gctatgtaca aagctccctg aaaccttgtc tctctaaagt tagtgtgcag
     2521 ggttttccaa ggctgagaga gcctaataca tggggaagca cttccttgag gtggaagatc
     2581 tctcccttca cctttcctct ttttccctgc aggctagtgc ctacttttta tcagtttgca
     2641 caatcgctta gataaacacc gaggaggaga ttctctttaa ttatcaaaga cacatctttt
     2701 cagggggcca acaaagcatt tatttcaccc gccaaactaa aggagagtta ttccagttta
     2761 ggaggaagag gcgcgccgag ctcggatcct gagaacttca gggtgagtct atgggaccct
     2821 tgatgttttc tttccccttc ttttctatgg ttaagttcat gtcataggaa ggggagaagt
     2881 aacagggtac acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc
     2941 tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc taatctcttt
     3001 ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta aagaataaca
     3061 gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt tctgcatata
     3121 aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt
     3181 ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc taggcccttt
     3241 tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct
     3301 gtgtgctggc ccatcacttt ggcaaagcac gtgggccgcc tcggccaccg gtcgccacca
     3361 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
     3421 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     3481 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     3541 tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg
     3601 actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     3661 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggctcc ttcatctaca
     3721 aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacta
     3781 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     3841 tccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct
     3901 acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca
     3961 tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcgcc gagggccgcc
     4021 accacctgtt cctgtagagt ttaaaccgta cgggaaacgg ccctattcta tagtgtcacc
     4081 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     4141 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4201 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4261 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcgcggcc gctctagagc
     4321 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga
     4381 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     4441 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgccagctgg
     4501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4561 gaatggaatt ccagacgatt gagcgtcaaa atgtaggtat ttccatgagc gtttttcctg
     4621 ttgcaatggc tggcggtaat attgttctgg atattaccag caaggccgat agtttgagtt
     4681 cttctactca ggcaagtgat gttattacta atcaaagaag tattgcgaca acggttaatt
     4741 tgcgtgatgg acagactctt ttactcggtg gcctcactga ttataaaaac acttctcagg
     4801 attctggcgt accgttcctg tctaaaatcc ctttaatcgg cctcctgttt agctcccgct
     4861 ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc
     4921 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4981 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5041 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5101 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5161 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5221 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5281 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5341 tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg
     5401 gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg
     5461 ttcatcgatt ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagag
     5521 acctctcaaa aatagctacc ctctccggca tgaatttatc agctagaacg gttgaatatc
     5581 atattgatgg tgatttgact gtctccggcc tttctcaccc gtttgaatct ttacctacac
     5641 attactcagg cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg
     5701 aaataaaggc ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt
     5761 tagctttatg ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg
     5821 atttattgga tgttggaatt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5881 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5941 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6001 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6061 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     6121 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6241 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6301 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6361 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6421 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6481 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6541 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6601 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6661 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6721 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6781 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6841 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6901 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6961 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7021 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7081 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7141 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7201 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7441 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7921 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7981 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8041 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8101 ctccccgcgc gttggccgat tcattaatg
//
