LOCUS       sequence-10511-e        5698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-DR1-NRSE-RFP
FEATURES             Location/Qualifiers
     source          1..5698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     promoter        275..313
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          372..847
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      923..932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             929..1606
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(1059..1077)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1109..1129)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1327..1346
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1521..1544
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(1636..1654)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1637..1654)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1674..1691)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1680..1791
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2482..2995
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2569..2588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2779..2800
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3476..3495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3595..3617
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3655..3673)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3741..3845
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3846..3914,3915..4706)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4064..4083)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4877..5465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5366..5385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacttcagca ccacggacag cgcccctgca
      241 ggtctagggg tcaaaggtca ccggtcttaa ttaaggtagg cgtgtacggt gggaggtcta
      301 tataagcaga gctggtttag tgaaccgtca gatccgctag cgcgccgagc tcggatcctg
      361 agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct tttctatggt
      421 taagttcatg tcataggaag gggagaagta acagggtaca catattgacc aaatcagggt
      481 aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc
      541 ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat
      601 gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg caatagcaat
      661 atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg
      721 ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg
      781 gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct
      841 cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg gcaaagcacg
      901 tgggccgcct cggccaccgg tcgccaccat ggcctcctcc gaggacgtca tcaaggagtt
      961 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1021 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1081 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
     1141 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
     1201 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1261 ctccctgcag gacggctcct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
     1321 cgacggcccc gtaatgcaga agaagactat gggctgggag gcctccaccg agcgcctgta
     1381 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg
     1441 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg
     1501 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt
     1561 ggagcagtac gagcgcgccg agggccgcca ccacctgttc ctgtagagtt taaaccgtac
     1621 gggaaacggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
     1681 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     1741 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1801 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1861 ggaagacaat agcgcggccg ctctagagca tggctacgta gataagtagc atggcgggtt
     1921 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     1981 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     2041 ctcagtgagc gagcgagcgc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     2101 cttcccaaca gttgcgcagc ctgaatggcg aatggaattc cagacgattg agcgtcaaaa
     2161 tgtaggtatt tccatgagcg tttttcctgt tgcaatggct ggcggtaata ttgttctgga
     2221 tattaccagc aaggccgata gtttgagttc ttctactcag gcaagtgatg ttattactaa
     2281 tcaaagaagt attgcgacaa cggttaattt gcgtgatgga cagactcttt tactcggtgg
     2341 cctcactgat tataaaaaca cttctcagga ttctggcgta ccgttcctgt ctaaaatccc
     2401 tttaatcggc ctcctgttta gctcccgctc tgattctaac gaggaaagca cgttatacgt
     2461 gctcgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     2521 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2581 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     2641 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2701 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     2761 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     2821 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     2881 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttaaa
     2941 tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata
     3001 tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct
     3061 caggcaatga cctgatagcc tttgtagaga cctctcaaaa atagctaccc tctccggcat
     3121 gaatttatca gctagaacgg ttgaatatca tattgatggt gatttgactg tctccggcct
     3181 ttctcacccg tttgaatctt tacctacaca ttactcaggc attgcattta aaatatatga
     3241 gggttctaaa aatttttatc cttgcgttga aataaaggct tctcccgcaa aagtattaca
     3301 gggtcataat gtttttggta caaccgattt agctttatgc tctgaggctt tattgcttaa
     3361 ttttgctaat tctttgcctt gcctgtatga tttattggat gttggaattc ctgatgcggt
     3421 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     3481 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3541 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3601 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     3661 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3841 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3901 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3961 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4021 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4081 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4141 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4201 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4261 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4321 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4381 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4441 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4501 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4561 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4621 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4681 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4741 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4801 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4861 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4921 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4981 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5041 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5101 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5161 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5221 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5281 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5341 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5401 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5461 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5521 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5581 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5641 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatg
//
