LOCUS       sequence-10512-e        5866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-E1.1-RFP
FEATURES             Location/Qualifiers
     source          1..5866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     primer_bind     complement(333..349)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        388..426
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          485..960
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     1042..1058
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1091..1100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1774
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(1227..1245)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1277..1297)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1495..1514
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1689..1712
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(1804..1822)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1805..1822)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1842..1859)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1848..1959
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2650..3163
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2737..2756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2947..2968
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3644..3663)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3763..3785
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3823..3841)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3909..4013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4014..4082,4083..4874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4232..4251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5045..5633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5534..5553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta tacggccggc cacgcgtgga tcctcattca
      241 cgcctagaag cagagatcct cattcacgcc tagaagcaga gatcctcatt cacgcctaga
      301 agcagagatc ctcattcacg cctagaagca gagatccact agttctagag cggccgccac
      361 cgacgagcta gcggcgcgat taattaaggt aggcgtgtac ggtgggaggt ctatataagc
      421 agagctggtt tagtgaaccg tcagatccgc tagcgcgccg agctcggatc ctgagaactt
      481 cagggtgagt ctatgggacc cttgatgttt tctttcccct tcttttctat ggttaagttc
      541 atgtcatagg aaggggagaa gtaacagggt acacatattg accaaatcag ggtaattttg
      601 catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt atcttatttc
      661 taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat catgcctctt
      721 tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc aatatttctg
      781 catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata ttgctaatag
      841 cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg ctggattatt
      901 ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt cctcccacag
      961 ctcctgggca acgtgctggt ctgtgtgctg gcccatcact ttggcaaagc acgtgaattc
     1021 gaagatctgg ccgcctcggc ctctagaact agtggatccc ccgggctgca ggaattcgat
     1081 gctaccggtc gccaccatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa
     1141 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1201 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     1261 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
     1321 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
     1381 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1441 cggctccttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
     1501 aatgcagaag aagactatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     1561 cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc actacctggt
     1621 ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
     1681 ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga
     1741 gcgcgccgag ggccgccacc acctgttcct gtagagttta aaccgtacgg gaaacggccc
     1801 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
     1861 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     1921 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     1981 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2041 cgcggccgct ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     2101 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     2161 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     2221 gcgagcgcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2281 tgcgcagcct gaatggcgaa tggaattcca gacgattgag cgtcaaaatg taggtatttc
     2341 catgagcgtt tttcctgttg caatggctgg cggtaatatt gttctggata ttaccagcaa
     2401 ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc aaagaagtat
     2461 tgcgacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc tcactgatta
     2521 taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt taatcggcct
     2581 cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc
     2641 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     2701 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2761 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2821 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2881 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2941 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3001 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3061 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaata tttgcttata
     3121 caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg attgacatgc
     3181 tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca ggcaatgacc
     3241 tgatagcctt tgtagagacc tctcaaaaat agctaccctc tccggcatga atttatcagc
     3301 tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt ctcacccgtt
     3361 tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg gttctaaaaa
     3421 tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg gtcataatgt
     3481 ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt ttgctaattc
     3541 tttgccttgc ctgtatgatt tattggatgt tggaattcct gatgcggtat tttctcctta
     3601 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     3661 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3721 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3781 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3841 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatg
//
