LOCUS       sequence-105615-        7480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the isoform IV of PML (promyelocytic leukemia protein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Vernier M, Bourdeau V, Gaumont-Leclerc MF, Moiseeva O, Begin V, Saad
            F, Mes-Masson AM, Ferbeyre G
  TITLE     Regulation of E2Fs and senescence by PML nuclear bodies.
  JOURNAL   Genes Dev. 2011 Jan 1;25(1):41-50. doi: 10.1101/gad.1975111.
  PUBMED    21205865
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-01-1- 1"; volume: "25"; issue:
            "1"; pages: "41-50"
COMMENT     Sequence Label: pLPC-Flag-PML-IV
FEATURES             Location/Qualifiers
     source          1..7480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1318..1334
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1367..1966
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1367..1386)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1863..1883
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        2302..2505
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2456..2476
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2550..2569
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2550..2568
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2603..2668
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5555..5572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5726..6314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5806..5825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6492..7283,7284..7352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7115..7134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7353..7457)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
     1321 tatccagccc tcactccttc tctaggcgcc ggccggatca gcttccatga ccgagtacaa
     1381 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     1441 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     1501 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     1561 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     1621 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     1681 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     1741 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     1801 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     1861 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgagtgccc
     1921 gaaggaccgc gcgacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     1981 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgat atcggcctgg ctgaccgccc
     2041 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2101 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2161 caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa atggcccgcc
     2221 tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta catctacgta
     2281 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag
     2341 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2401 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa
     2461 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     2521 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
     2581 tggtaccgag ctcggatcca tggactacaa agaccatgac ggtgattata aagatcatga
     2641 catcgattac aaggatgacg atgacaagct tgcggccgcg aattcaatgg agcctgcacc
     2701 cgcccgatct ccgaggcccc agcaggaccc cgcccggccc caggagccca ccatgcctcc
     2761 ccccgagacc ccctctgaag gccgccagcc cagccccagc cccagcccta cagagcgagc
     2821 ccccgcttcg gaggaggagt tccagtttct gcgctgccag caatgccagg cggaagccaa
     2881 gtgcccgaag ctgctgcctt gtctgcacac gctgtgctca ggatgcctgg aggcgtcggg
     2941 catgcagtgc cccatctgcc aggcgccctg gcccctaggt gcagacacac ccgccctgga
     3001 taacgtcttt ttcgagagtc tgcagcggcg cctgtcggtg taccggcaga ttgtggatgc
     3061 gcaggctgtg tgcacccgct gcaaagagtc ggccgacttc tggtgctttg agtgcgagca
     3121 gctcctctgc gccaagtgct tcgaggcaca ccagtggttc ctcaagcacg aggcccggcc
     3181 cctagcagag ctgcgcaacc agtcggtgcg tgagttcctg gacggcaccc gcaagaccaa
     3241 caacatcttc tgctccaacc ccaaccaccg cacccctacg ctgaccagca tctactgccg
     3301 aggatgttcc aagccgctgt gctgctcgtg cgcgctcctt gacagcagcc acagtgagct
     3361 caagtgcgac atcagcgcag agatccagca gcgacaggag gagctggacg ccatgacgca
     3421 ggcgctgcag gagcaggata gtgcctttgg cgcggttcac gcgcagatgc acgcggccgt
     3481 cggccagctg ggccgcgcgc gtgccgagac cgaggagctg atccgcgagc gcgtgcgcca
     3541 ggtggtagct cacgtgcggg ctcaggagcg cgagctgctg gaggctgtgg acgcgcggta
     3601 ccagcgcgac tacgaggaga tggccagtcg gctgggccgc ctggatgctg tgctgcagcg
     3661 catccgcacg ggcagcgcgc tggtgcagag gatgaagtgc tacgcctcgg accaggaggt
     3721 gctggacatg cacggtttcc tgcgccaggc gctctgccgc ctgcgccagg aggagcccca
     3781 gagcctgcaa gctgccgtgc gcaccgatgg cttcgacgag ttcaaggtgc gcctgcagga
     3841 cctcagctct tgcatcaccc aggggaaaga tgcagctgta tccaagaaag ccagcccaga
     3901 ggctgccagc actcccaggg accctattga cgttgacctg cccgaggagg cagagagagt
     3961 gaaggcccag gttcaggccc tggggctggc tgaagcccag cctatggctg tggtacagtc
     4021 agtgcccggg gcacaccccg tgccagtgta cgccttctcc atcaaaggcc cttcctatgg
     4081 agaggatgtc tccaatacaa cgacagccca gaagaggaag tgcagccaga cccagtgccc
     4141 caggaaggtc atcaagatgg agtctgagga ggggaaggag gcaaggttgg ctcggagctc
     4201 cccggagcag cccaggccca gcacctccaa ggcagtctca ccaccccacc tggatggacc
     4261 gcctagcccc aggagccccg tcataggaag tgaggtcttc ctgcccaaca gcaaccacgt
     4321 ggccagtggc gccggggagg cagaggaacg cgttgtggtg atcagcagct cggaagactc
     4381 agatgccgaa aactcgtcct cccgagagct ggatgacagc agcagtgagt ccagtgacct
     4441 ccagctggaa ggccccagca ccctcagggt cctggacgag aaccttgctg acccccaagc
     4501 agaagacaga cctctggttt tctttgacct caagattgac aatgaaagtg ggttctcctg
     4561 gggctacccc cacccctttc taatttagtc tctgagtccc aaagaattaa ttctgcagat
     4621 atccatcaca ctggcggccg gttaacctcg agcatgcatc tagagggccg ataaaataaa
     4681 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     4741 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     4801 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     4861 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     4921 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     4981 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     5041 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     5101 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     5161 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     5221 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     5281 tacccgtcag ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     5341 gggggtcttt catttccgac ttgtggtctc gctgccttgg gagggtctcc tctgagtgat
     5401 tgactacccg tcagcggggg tcttcacatg cagcatgtat caaaattaat ttggtttttt
     5461 ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg
     5521 agaggcggtt tgcgtattgg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     6181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6421 ttttaaatta aaaatgaagt ttgcggccgc aaatcaatct aaagtatata tgagtaaact
     6481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7081 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     7141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7441 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
