LOCUS       sequence-105655-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB109
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(172..450)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc cataattttt
       61 ggcagaggga aaaagatctc agtggtattt gtgagccagg gcattggcca caccagccac
      121 caccttctga taggcagcct gcacctgagt gtttacttgt acttatctag tctttgagat
      181 tcgtcggaac acatgataat agaggtccca ggcttggagg aggtccttga cattccctga
      241 tttcatgtac ttcctgcacc actcttgggc ctccattaaa tctcgaccat aggcctgatt
      301 aaaggatgtt tccttcagag tctgggggcc ccgttccatc atagcatgca agggctccag
      361 cacctcaaac atgcctttca cgttcctttc cccaaagtac aaacgagatg cctcttccag
      421 gccttcatgc cacatctcat gccagaggat tctagatccg gtggatcccg ggcccgcggt
      481 acccttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      541 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
      601 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
      661 gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttca
//
