LOCUS       sequence-105656-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB109
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    43..91
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        164..268
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(487..506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac
       61 ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc
      121 tccttccgtg tttattaagc ttggcacttt tcggggaaat gtgcgcggaa cccctatttg
      181 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      241 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      301 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      361 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      421 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      481 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      541 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      601 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      661 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg c
//
