LOCUS       sequence-105660-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB127
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(112..390)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     primer_bind     complement(437..456)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     ncRNA           complement(531..572)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(598..624)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat
       61 ggccatgtta tcctcctcgc ccttgctcac catggtggcg accggactag tctttgagat
      121 tcgtcggaac acatgataat agaggtccca ggcttggagg aggtccttga cattccctga
      181 tttcatgtac ttcctgcacc actcttgggc ctccattaaa tctcgaccat aggcctgatt
      241 aaaggatgtt tccttcagag tctgggggcc ccgttccatc atagcatgca agggctccag
      301 cacctcaaac atgcctttca cgttcctttc cccaaagtac aaacgagatg cctcttccag
      361 gccttcatgc cacatctcat gccagaggat tctagaagcc atggtgaccg gtggatcccg
      421 ggcccgcggt accgacacct gtggagagaa aggcaaagtg gatgtcagta agaccaatag
      481 gtgcctatct ggtccacgcg tatgcgatat cagcagctag cgccgaattc ggccatttgt
      541 tccatgtgag tgctagtaac aggccttgtg tcctgtgaga cgcatctgta cgtctctagc
      601 atacagcctt cagcaagcct ccagagctct agaagtcgac cgaaacgcaa gagtcttctc
      661 tgtctcgaca agcccagttt ctattggtct
//
