LOCUS       sequence-105663-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB127
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    10..58
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    270..303
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     ncRNA           459..485
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           549..590
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     665..684
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
       61 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
      121 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
      181 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt
      241 tagggacggg gtcccccatt cgagaataaa taacttcgta taaagtatcc tatacgaagt
      301 tatcaccctg cagtacgtat ccggtagcct tgcagaagtt ggtcgtgagg cactgggcag
      361 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
      421 cagagaagac tcttgcgttt cggtcgactt ctagagctct ggaggcttgc tgaaggctgt
      481 atgctgatat ggattcatgt gcacatggtt ttggccactg actgaccatg tgcatgaatc
      541 catatcagga cacaaggcct gttactagca ctcacatgga acaaatggcc gaattcggcg
      601 ctagctgctg atatcgcata cgcgtggacc agataggcac ctattggtct tactgacatc
      661 cactttgcct ttctctccac aggtgtcggt accgc
//
