LOCUS       sequence-105675-        3216 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human RNF31/HOIP codon optimized for E. coli and 
            insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Smit JJ, Monteferrario D, Noordermeer SM, van Dijk WJ, van der 
            Reijden BA, Sixma TK
  TITLE     The E3 ligase HOIP specifies linear ubiquitin chain assembly through
            its RING-IBR-RING domain and the unique LDD extension.
  JOURNAL   EMBO J. 2012 Oct 3;31(19):3833-44. doi: 10.1038/emboj.2012.217. Epub
            2012 Aug 3.
  PUBMED    22863777
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.217"; journalName: "EMBO J"; date: "2012-10-3- 
            3"; volume: "31"; issue: "19"; pages: "3833-44"
COMMENT     Sequence Label: pGEX6p1-GST-Rnf31
FEATURES             Location/Qualifiers
     source          1..3216
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcctggcg aagaagaaga acgtgcattc ctggttgccc gtgaagaact ggcgtcggct
       61 ctgcgtcgtg attccggtca agcgttttct ctggaacagc tgcgcccgct gctggctagc
      121 tccctgcctc tggctgctcg ttatctgcag ctggatgctg cacgtctggt gcgttgcaat
      181 gcacacggcg agccccgcaa ctatctgaat accctgagta ctgccctgaa tatcctggaa
      241 aagtacggtc gtaacctgct gtcgccacag cgcccgcgtt attggcgcgg cgtcaaattc
      301 aacaatccgg tttttcgttc aaccgtggat gctgtccagg gtggccgcga tgtcctgcgt
      361 ctgtacggct ataccgaaga gcaaccagac ggactgtcat tcccggaggg tcaggaagag
      421 cctgatgagc accaagttgc cacggtgacc ctggaagttc tgctgctgcg caccgagctg
      481 tctctgctgc tgcagaacac tcatccccgt cagcaagctc tggaacaact gctggaggac
      541 aaggtggaag acgatatgct gcagctgagc gaattcgatc cactgctgcg cgagattgcg
      601 cccggtccac tgaccactcc tagtgtcccc ggctcgactc cgggaccttg ctttctgtgt
      661 ggttctgcac cgggaaccct gcactgccca tcatgtaaac aggccctgtg ccccgcttgt
      721 gaccatctgt tccacggtca tccatcgcgc gctcaccatc tgcgtcagac tctgccggga
      781 gtgctgcagg gtacgcatct gtctccatct ctgcctgcat ctgcacagcc ccgtccacaa
      841 agtacctcgc tgctggctct gggcgattct tcactgagtt cgccgaatcc tgcttccgcg
      901 cacctgcctt ggcattgcgc cgcttgtgcg atgctgaacg agccgtgggc ggtcctgtgc
      961 gttgcatgtg accgccctcg tggttgcaag ggactgggtc tgggaaccga aggtccacag
     1021 ggaactggag gtctggaacc ggatctggca cgcggccgtt gggcttgcca gtcttgtacc
     1081 tttgaaaacg aggcggcagc cgttctgtgc tcaatctgtg aacgcccgcg tctggcacag
     1141 ccgcctagcc tggtggtgga ctcccgcgat gctggtattt gcctgcaacc tctgcagcaa
     1201 ggtgatgctc tgctggcatc tgcacagtcc caagtctggt actgcatcca ctgtaccttc
     1261 tgcaatagct ccccgggctg ggtctgtgtt atgtgcaacc gcacttcttc acccattcca
     1321 gcccagcatg ctccgcgtcc ttatgctagt tcgctggaaa aaggtccccc aaaaccgggc
     1381 ccgcctcgtc gcctgtctgc gcccctgcca agctcctgcg gagacccaga aaagcagcgc
     1441 caagataaaa tgcgtgaaga gggtctgcag ctggtttcaa tgattcgtga aggtgaagct
     1501 gctggagcat gccctgaaga gatcttcagc gctctgcagt actcaggtac cgaagtgccg
     1561 ctgcaatggc tgcgttcaga gctgccttat gtgctggaga tggtcgcgga actggctggt
     1621 cagcaagatc cgggactggg tgccttcagt tgtcaggaag cccgtcgcgc ttggctggac
     1681 cgtcatggaa acctggatga agctgttgaa gagtgcgtgc gtacccgtcg ccgtaaggtc
     1741 caggaactgc aaagtctggg ctttggacct gaagagggct cgctgcaggc tctgtttcaa
     1801 catggcggag acgttagccg tgcactgacc gaactgcagc gccaacgtct ggagcctttt
     1861 cgccagcgtc tgtgggattc tggacctgag cctactccat cctgggacgg tcctgataaa
     1921 cagtctctgg tgcgccgtct gctggctgtc tatgcactgc catcttgggg tcgtgctgaa
     1981 ctggctctgt ccctgctgca ggagacccca cgtaattatg aactgggcga cgttgtggag
     2041 gcggttcgcc acagccagga tcgtgcattc ctgcgccgtc tgctggcgca agaatgtgca
     2101 gtgtgcggtt gggccctgcc gcataaccgc atgcaggctc tgacttcctg cgagtgtacg
     2161 atctgtcctg actgcttccg tcaacacttt acgatcgcac tgaaggaaaa acatattacc
     2221 gacatggtgt gtcctgcctg cggtcgtccc gatctgacgg acgataccca gctgctgtct
     2281 tacttctcaa cgctggacat ccaactgcgt gaatctctgg agccggatgc ttatgcgctg
     2341 ttccacaaga aactgactga gggcgtgctg atgcgcgatc ctaaatttct gtggtgtgct
     2401 cagtgcagct tcggatttat ttacgaacgt gagcaactgg aagcgacgtg tccgcagtgc
     2461 caccaaacct tctgtgtccg ctgcaagcgt cagtgggaag agcaacatcg cggtcgtagc
     2521 tgcgaagact ttcagaactg gaaacgcatg aatgatcccg agtaccaggc ccaaggcctg
     2581 gctatgtatc tgcaggaaaa cggaattgat tgtccaaagt gcaaattctc ttatgcactg
     2641 gcacgcggtg gctgcatgca ctttcattgt acccagtgcc gtcaccaatt ctgttccggt
     2701 tgctacaacg cgttttatgc aaagaataaa tgtccagagc cgaactgccg cgtgaagaaa
     2761 tctctgcatg gccaccatcc gcgtgactgc ctgttctatc tgcgcgattg gactgccctg
     2821 cgtctgcaga agctgctgca agacaacaat gtcatgttta atacggagcc cccagctggt
     2881 gctcgtgcag ttcccggagg tggctgccgt gttatcgaac agaaggaagt gccaaacggc
     2941 ctgcgcgatg aggcatgtgg aaaagaaacc cctgctggat atgctggtct gtgccaggcc
     3001 cactacaaag agtatcttgt gagtctgatt aacgcgcatt cgctggaccc ggcaaccctg
     3061 tacgaagtgg aagagctgga gactgctacg gaacgctatc tgcatgtccg tcctcagccg
     3121 ctggctggag aagatccgcc tgcttaccaa gcacgtctgc tgcaaaaact gaccgaagaa
     3181 gtgcccctgg gacagagtat tcctcgtcgt cgcaag
//
