LOCUS       sequence-10568-e       17314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Xu L, Blackburn EH
  TITLE     Human Rif1 protein binds aberrant telomeres and aligns along 
            anaphase midzone microtubules.
  JOURNAL   J Cell Biol. 2004 Dec 6. 167(5):819-30.
  PUBMED    15583028
REFERENCE   2  (bases 1 to 17314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2004 Dec 6. 167(5):819-30."
COMMENT     Sequence Label: pHR'CMVGFP_hRIF1(406-2446)IREShygro
FEATURES             Location/Qualifiers
     source          1..17314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             3..236
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(261..279)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(262..279)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(569..588)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(802..821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     921..943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(981..999)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1067..1171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1172..1240,1241..2032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1390..1409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2203..2791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2692..2711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2945..2962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3032..3052)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3037..3366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3217..3352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4500..4565
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4695..4715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5140..5274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5177..5196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5231..5250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5443..6076
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6123..6248
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6741..6974
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7159..7203
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        7482..7785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7786..7989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7940..7960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7986..8010
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8079..8088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8085..8087,8088..8090,8091..8801)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8085..8087,8088..8090,8091..8801)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8130..8151)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8391..8410)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8738..8759
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    15073..15622
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(15217..15234)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     15444..15463
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             15625..16650
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             15625..16650
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    16676..17264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(16729..16749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(17147..17158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
       61 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      121 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      181 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
      241 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
      301 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
      361 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
      421 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
      481 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
      541 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      601 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      661 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      721 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      781 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      841 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      901 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      961 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1021 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1081 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1141 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1201 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1261 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1321 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1381 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1441 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1501 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1561 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1621 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1681 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1741 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1801 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1861 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1921 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1981 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2041 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2101 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2161 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2221 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2281 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2341 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2401 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2461 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2521 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2581 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2641 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2701 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2761 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2821 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2881 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2941 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3001 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     3061 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3361 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     3421 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     3481 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     3541 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     3601 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     3661 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     3721 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     3781 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     3841 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     3901 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     3961 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     4021 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     4081 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     4141 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     4201 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     4261 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     4321 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     4381 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     4441 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     4501 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     4561 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     4621 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     4681 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     4741 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     4801 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     4861 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     4921 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     4981 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     5041 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     5101 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     5161 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5221 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     5281 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     5341 aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     5401 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     5461 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5521 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     5581 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     5641 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     5701 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     5761 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     5821 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     5881 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5941 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6001 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6061 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     6121 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     6181 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     6241 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     6301 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     6361 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     6421 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     6481 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     6541 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     6601 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     6661 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     6721 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     6781 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     6841 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     6901 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     6961 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     7021 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     7081 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     7141 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     7201 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     7261 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     7321 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     7381 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     7441 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
     7501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     7561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     7621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     7681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     7741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     7801 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     7861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     7921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     7981 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     8041 ctccatagaa gacaccgact ctagctagag gatccgtcgc caccatggtg agcaagggcg
     8101 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     8161 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     8221 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     8281 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     8341 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     8401 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     8461 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     8521 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     8581 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     8641 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     8701 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     8761 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatccatgg
     8821 cttcctcccc gtacggagcc ccgggaactc cccgaatgaa cctgagttcg aatttaggtg
     8881 gaatggccac aatcccatcc attcaacttt tgggacttga aatgttgctt catttcttgt
     8941 tgggtccaga agccttgagt tttgctaagc aaaataaact tgtgctgagc ttagagccat
     9001 tggaacatcc gttaatcagc agcccttcct ttttttccaa acatgcaaat acacttatca
     9061 ctgctgttca tgatagcttt gttgcagttg gaaaagatgc ccccgatgtg gttgtcagtg
     9121 ctatctggaa ggagctaatt agcttggtga agtcagttac tgaatcaggt aacaaaaaag
     9181 agaaaccagg ttctgaagtt ttgactctct tattaaagtc tttggaaagc atagtaaagt
     9241 ctgaagtatt tcctgtatca aaaacgctgg tcctcatgga aattacaatt aaaggacttc
     9301 ctcagaaagt attaggttca ccagcatatc aggttgctaa tatggatatt cttaatggaa
     9361 ctccagcttt gttcttaatt caattaattt tcaacaattt cttggaatgt ggtgtatcag
     9421 atgaaaggtt ctttctcagt ttggaatcac ttgtaggctg tgttctttct ggtccaactt
     9481 caccactagc tttcagtgac tcagttttaa atgttattaa tcaaaatgca aagcagttgg
     9541 aaaataagga gcatctctgg aaaatgtgga gtgttatagt caccccatta actgaattga
     9601 ttaatcagac caatgaagta aatcaaggtg atgccttaga acataatttt agtgccatct
     9661 atggtgcatt gactttacca gtaaaccaca ttttttcaga acagagattt ccagtggcca
     9721 ccatgaagac tttgcttaga acttggtcag aattatatag agcatttgct cgttgtgctg
     9781 ctttggtggc aacagcagaa gagaacttgt gctgtgagga actttcttcc aagataatgt
     9841 ccagtttgga agatgaaggc ttttctaatt tgttgttcgt ggatagaatt atttatatta
     9901 ttactgtaat ggttgattgc attgacttct caccatataa tattaaatat cagcccaaag
     9961 ttaaatcacc acagagacct tcagattggt ccaaaaagaa gaatgagccc ctagggaaat
    10021 tgacttcttt atttaaactt attgtgaaag tgatctattc tttccacaca ctgagcttca
    10081 aggaagcaca ttctgatacc ctcttcacta ttggcaactc aatcaccagc attatttcca
    10141 gtgtacttgg gcatatttct ttgccttcta tgatccgaaa aatatttgca actttaacaa
    10201 gacctctggc attattttat gaaaactcaa agcttgatga agttcctaaa gtatatagtt
    10261 gtctgaacaa caagttagaa aagctactgg gagaaattat tgcttgtctg caattcagct
    10321 acaccggaac ttatgatagt gaacttcttg aacaactctc cccactatta tgcataatat
    10381 ttctgcacaa gaataaacag attcgaaaac agagtgctca gttctggaat gccacttttg
    10441 ccaaagtgat gatgttggtt tatcctgaag agttaaaacc agtactaaca caagccaaac
    10501 aaaaatttct gctcctgttg cctggtttgg aaactgttga aatgatggag gaatccagtg
    10561 gaccatattc tgatggaaca gaaaattcac aactaaatgt gaagataagt ggcatggaga
    10621 gaaaatcaaa tggaaaaaga gattcatttt tggcacaaac aaagaataaa aaagaaaata
    10681 tgaaaccagc agccaaactg aaacttgaat cttcgtcttt aaaagtaaag ggtgaaattc
    10741 ttttggaaga ggaaaagtct actgactttg tgtttatacc tccagaagga aaagatgcaa
    10801 aggaaagaat attaactgat catcaaaaag aagttctcaa aacaaagcgg tgtgatattc
    10861 ctgccatgta taataatctg gatgtttccc aagatacctt atttactcag tatagtcagg
    10921 aagagcctat ggaaattcct actttaacca gaaaaccaaa ggaggattct aagatgatga
    10981 ttacggagga gcaaatggac agtgacattg tcattcctca agatgtcacg gaagactgtg
    11041 gtatggctga acatcttgaa aagtcctccc tttcgaataa tgagtgtggt tctcttgaca
    11101 aaaccagtcc agaaatgtca aacagtaata atgatgaaag aaaaaaagct ttaatttcat
    11161 caaggaaaac atcaactgaa tgtgcatcta gtacagaaaa ttctttcgtt gtcagcagta
    11221 gttcagtttc taataccact gttgctggaa ctcccccata ccctacaagt cggaggcaaa
    11281 cctttattac tttggagaag tttgatggtt cagaaaatag accttttagt ccatccccct
    11341 tgaataatat ttcatcaact gttacagtga aaaataacca ggaaaccatg attaaaacag
    11401 attttctacc aaaagcaaag caaagagaag ggactttttc aaaatctgat tctgaaaaaa
    11461 tagtgaatgg aactaagaga tcaagccgga gagctggtaa agctgaacaa acagggaata
    11521 aaaggtctaa gcccttaatg agatctgagc cggagaaaaa tactgaggaa tctgttgaag
    11581 gcattgtagt cttagaaaat aacccacctg gtttgcttaa tcaaacagaa tgtgtgtcag
    11641 ataatcaggt tcatctttct gaatctacaa tggagcatga caatacaaag cttaaagcag
    11701 caacaatgga aaatgctgta ttattggaaa ctaatactgt agaggagaaa aatgtagaaa
    11761 ttaatttgga atccaaagag aatacacccc cagtagtaat atcagcagat caaatggtaa
    11821 atgaggatag tcaggttcag ataactccaa atcagaaaac ccttagacgg tcttcaaggc
    11881 gacgttcaga agtagtagag tctaccactg aaagccaaga taaggaaaat agtcatcaaa
    11941 aaaaggaacg acgtaaggaa gaagaaaaac ctcttcagaa gagtccattg catataaaag
    12001 atgatgtgtt acctaaacaa aaactgattg ctgaacaaac tctacaggag aatttaattg
    12061 agaaaggaag taatttacat gagaagactc ttggggaaac tagtgctaat gcagaaactg
    12121 aacaaaataa aaaaaaggca gaccctgaga acattaagtc tgagggggat ggtacccagg
    12181 acattgtaga taagtcctct gagaaactag tcagaggccg aacacggtat caaactagaa
    12241 gagcatctca gggtttgctt tccagcattg aaaactcaga atctgatagt tcggaggcaa
    12301 aagaagaagg ttctaggaag aagagatctg gaaaatggaa aaacaaaagc aatgaaagtg
    12361 ttgacattca agatcaagaa gagaaagtgg tgaaacagga atgtataaaa gctgaaaatc
    12421 agtcacatga ttataaagca acttctgaag aagatgtaag cataaaatct ccgatttgcg
    12481 aaaaacaaga tgaaagtaat actgtaatat gtcaggattc tacagtaact tcagatttgt
    12541 tgcaagttcc tgatgattta ccaaatgtgt gtgaggaaaa aaatgaaact agcaaatatg
    12601 cagaatattc ctttacaagt ctacctgtgc cagaatcaaa tctaaggact agaaatgcca
    12661 ttaagagatt acataagcga gactcttttg ataattgtag tttgggagaa tcctcaaaaa
    12721 tagggatatc agatatttct tcgctttcag aaaaaacttt tcaaacactt gaatgccaac
    12781 acaagagaag taggagggtg aggagatcta aaggttgtga ttgctgtggg gaaaaatcac
    12841 aacctcagga aaagtcactc attgggttaa agaatacaga aaataatgac gtagagatta
    12901 gtgaaacaaa aaaggcagat gtgcaagcac ctgtaagccc atcagaaact tctcaagcta
    12961 atccatattc tgaaggacaa tttttagatg aacatcatag tgtgaatttt catttgggtc
    13021 tcaaagagga taatgatact attaatgatt cattaattgt ttctgaaacc aaatcaaaag
    13081 aaaacactat gcaagaatct cttccttctg gaatagtaaa ctttagagag gaaatttgtg
    13141 atatggattc tagtgaagca atgtctcttg aaagccagga gtcacctaat gaaaatttta
    13201 aaactgttgg cccgtgttta ggagactcga aaaatgtttc acaggaatct ttggagacaa
    13261 aagaagaaaa accagaagaa accccaaaaa tggaactgag tctagagaat gttactgttg
    13321 aaggaaatgc atgtaaagta acagaatcca atctagagaa agcaaaaact atggaattga
    13381 atgtaggaaa tgaagctagc tttcatggac aagagagaac caaaactggt atttctgaag
    13441 aagcagcaat agaagaaaat aaaagaaatg atgactctga agcagacaca gctaaactga
    13501 atgccaaaga agtagcaact gaggaattta attcagatat tagtctttct gataatacta
    13561 cacctgtaaa attgaatgct caaactgaga tttctgaaca aacagcagct ggggaactag
    13621 atggaggaaa tgatgtatct gatctacact catctgaaga aacgaatacc aaaatgaaaa
    13681 attatgaaga aatgatgatc ggcgaggcaa tggctgaaac tggccatgat ggtgaaacag
    13741 agaatgaggg cataactacc aaaacctcaa agcctgatga agctgaaaca aacatgttga
    13801 ctgcagaaat ggacaacttt gtttgtgaca cagttgaaat gagcactgaa gaaggaatca
    13861 ttgacgctaa taaaactgaa acaaatactg agtatagtaa atctgaagaa aaattagata
    13921 acaatcaaat ggtaatggaa agtgatattt tacaggaaga tcaccatact tcacagaaag
    13981 tggaggaacc atcacagtgt ctggcatctg gaacagctat ctctgagcta ataatagaag
    14041 acaataatgc atctcctcaa aaactaaggg aacttgatcc ttcacttgtg tcagcaaatg
    14101 acagtcctag tggcatgcag acacgctgtg tctggtctcc tttggcttct ccgtctacga
    14161 gcattttaaa gagaggacta aaaagatccc aagaagatga aatctcatca cctgttaata
    14221 aggttcgccg tgtctccttt gcagatccaa tataccaagc aggattggca gatgacattg
    14281 atagacggtg ctctattgtt aggtcccatt cttccaatag ttctcccata ggaaaaagtg
    14341 ttaaaacttc tcctactaca caatctaagc ataataccac ttcagccaaa ggatttctgt
    14401 ccccaggatc acgtagccct aaatttaaga gctcaaagaa gtgtttaatt tcagaaatgg
    14461 ccaaagaatc cataccatgc ccaacagaaa gtgtttaccc accattggtg aactgtgtgg
    14521 caccagttga catcatttta cctcagatta catcaaacat gtgggcaaga ggcctgggac
    14581 aactcattag agctaagaat ataaaaacta ttggtgattt gagtactctt acagcatctg
    14641 aaataaaaac tcttcctatc cgttctccaa aagtgtccaa tgtaaaaaag gctctcagaa
    14701 tatatcatga gcagcaggtg aagactcgtg gactagaaga gattccagtt tttgatattt
    14761 ctgaaaaaac agtaaatgga atagaaaata aatctttgtc acctgatgaa gaaagacttg
    14821 tctcagatat aattgatcct gttgctttag aaattccatt atccaaaaac cttgtggcac
    14881 agattagtgc tcttgctctt cagctggatt cagaagatct tcataattat tcaggaagcc
    14941 aactatttga aatgcacgag aaactaagtt gtatggcaaa ctctgtaata aaaaatctac
    15001 agtcacgttg gagatcacca tcccatgaaa attctattta gctcgagcgg gatcaattcc
    15061 gccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
    15121 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
    15181 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
    15241 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
    15301 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
    15361 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
    15421 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
    15481 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
    15541 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
    15601 tcctttgaaa aacacgataa taccatgaaa aagcctgaac tcaccgcgac gtctgtcgag
    15661 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
    15721 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
    15781 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
    15841 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
    15901 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
    15961 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
    16021 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
    16081 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
    16141 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
    16201 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
    16261 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
    16321 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
    16381 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
    16441 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
    16501 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
    16561 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
    16621 cccagcactc gtccgagggc aaaggaatag gtcgagctca agcttcgaat tcgataatca
    16681 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
    16741 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
    16801 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
    16861 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
    16921 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
    16981 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
    17041 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
    17101 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
    17161 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
    17221 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattcgagct
    17281 cggtaccttt aagaccaatg acttacaagg cagc
//
