LOCUS       sequence-10569-e       14464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14464)
  AUTHORS   Xu L, Blackburn EH
  TITLE     Human Rif1 protein binds aberrant telomeres and aligns along 
            anaphase midzone microtubules.
  JOURNAL   J Cell Biol. 2004 Dec 6. 167(5):819-30.
  PUBMED    15583028
REFERENCE   2  (bases 1 to 14464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2004 Dec 6. 167(5):819-30."
COMMENT     Sequence Label: pHR'CMVGFP_hRIF1(1355-2446)IREShygro
FEATURES             Location/Qualifiers
     source          1..14464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             3..236
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(261..279)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(262..279)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(569..588)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(802..821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     921..943
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(981..999)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1067..1171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1172..1240,1241..2032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1390..1409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2203..2791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2692..2711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2945..2962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3032..3052)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3037..3366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3217..3352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4500..4565
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4695..4715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5140..5274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5177..5196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5231..5250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5443..6076
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6123..6248
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6741..6974
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7159..7203
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        7482..7785
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7786..7989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7940..7960
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7986..8010
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8079..8088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8085..8087,8088..8090,8091..8801)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8085..8087,8088..8090,8091..8801)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8130..8151)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8391..8410)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8738..8759
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    12223..12772
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12367..12384)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12594..12613
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12775..13800
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             12775..13800
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    13826..14414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13879..13899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14297..14308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
       61 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      121 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      181 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
      241 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
      301 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
      361 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
      421 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
      481 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
      541 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      601 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      661 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      721 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      781 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      841 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      901 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      961 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1021 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1081 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1141 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1201 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1261 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1321 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1381 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1441 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1501 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1561 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1621 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1681 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1741 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1801 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1861 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1921 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1981 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2041 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2101 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2161 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2221 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2281 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2341 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2401 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2461 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2521 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2581 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2641 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2701 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2761 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2821 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2881 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2941 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3001 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     3061 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3361 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     3421 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     3481 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     3541 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     3601 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     3661 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     3721 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     3781 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     3841 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     3901 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     3961 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     4021 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     4081 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     4141 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     4201 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     4261 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     4321 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     4381 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     4441 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     4501 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     4561 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     4621 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     4681 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     4741 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     4801 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     4861 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     4921 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     4981 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     5041 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     5101 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     5161 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5221 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     5281 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     5341 aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     5401 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     5461 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5521 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     5581 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     5641 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     5701 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     5761 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     5821 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     5881 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5941 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6001 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6061 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     6121 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     6181 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     6241 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     6301 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     6361 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     6421 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     6481 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     6541 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     6601 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     6661 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     6721 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     6781 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     6841 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     6901 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     6961 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     7021 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     7081 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     7141 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     7201 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     7261 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     7321 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     7381 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     7441 gtgaacggat ctcgacggta tcgataagct tgggagttcc gcgttacata acttacggta
     7501 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     7561 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     7621 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     7681 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     7741 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     7801 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     7861 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     7921 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     7981 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     8041 ctccatagaa gacaccgact ctagctagag gatccgtcgc caccatggtg agcaagggcg
     8101 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     8161 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     8221 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     8281 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     8341 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     8401 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     8461 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     8521 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     8581 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     8641 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     8701 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     8761 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggccgg actcagatcc
     8821 cagagcatga caatacaaag cttaaagcag caacaatgga aaatgctgta ttattggaaa
     8881 ctaatactgt agaggagaaa aatgtagaaa ttaatttgga atccaaagag aatacacccc
     8941 cagtagtaat atcagcagat caaatggtaa atgaggatag tcaggttcag ataactccaa
     9001 atcagaaaac ccttagacgg tcttcaaggc gacgttcaga agtagtagag tctaccactg
     9061 aaagccaaga taaggaaaat agtcatcaaa aaaaggaacg acgtaaggaa gaagaaaaac
     9121 ctcttcagaa gagtccattg catataaaag atgatgtgtt acctaaacaa aaactgattg
     9181 ctgaacaaac tctacaggag aatttaattg agaaaggaag taatttacat gagaagactc
     9241 ttggggaaac tagtgctaat gcagaaactg aacaaaataa aaaaaaggca gaccctgaga
     9301 acattaagtc tgagggggat ggtacccagg acattgtaga taagtcctct gagaaactag
     9361 tcagaggccg aacacggtat caaactagaa gagcatctca gggttttctt tccagcattg
     9421 aaaactcaga atctgatagt tcggaggcaa aagaagaagg ttctaggaag aagagatctg
     9481 gaaaatggaa aaacaaaagc aatgaaagtg ttgacattca agatcaagaa gagaaagtgg
     9541 tgaaacagga atgtataaaa gctgaaaatc agtcacatga ttataaagca acttctgaag
     9601 aagatgtaag cataaaatct ccgatttgcg aaaaacaaga tgaaagtaat actgtaatat
     9661 gtcaggattc tacagtaact tcagatttgt tgcaagttcc tgatgattta ccaaatgtgt
     9721 gtgaggaaaa aaatgaaact agcaaatatg cagaatattc ctttacaagt ctacctgtgc
     9781 cagaatcaaa tctaaggact agaaatgcca ttaagagatt acataagcga gactcttttg
     9841 ataattgtag tttgggagaa tcctcaaaaa tagggatatc agatatttct tcgctttcag
     9901 aaaaaacttt tcaaacactt gaatgccaac acaagagaag taggagggtg aggagatcta
     9961 aaggttgtga ttgctgtggg gaaaaatcac aacctcagga aaagtcactc attgggttaa
    10021 agaatacaga aaataatgac gtagagatta gtgaaacaaa aaaggcagat gtgcaagcac
    10081 ctgtaagccc atcagaaact tctcaagcta atccatattc tgaaggacaa tttttagatg
    10141 aacatcatag tgtgaatttt catttgggtc tcaaagagga taatgatact attaatgatt
    10201 cattaattgt ttctgaaacc aaatcaaaag aaaacactat gcaagaatct cttccttctg
    10261 gaatagtaaa ctttagagag gaaatttgtg atatggattc tagtgaagca atgtctcttg
    10321 aaagccagga gtcacctaat gaaaatttta aaactgttgg cccgtgttta ggagactcga
    10381 aaaatgtttc acaggaatct ttggagacaa aagaagaaaa accagaagaa accccaaaaa
    10441 tggaactgag tctagagaat gttactgttg aaggaaatgc atgtaaagta acagaatcca
    10501 atctagagaa agcaaaaact atggaattga atgtaggaaa tgaagctagc tttcatggac
    10561 aagagagaac caaaactggt atttctgaag aagcagcaat agaagaaaat aaaagaaatg
    10621 atgactctga agcagacaca gctaaactga atgccaaaga agtagcaact gaggaattta
    10681 attcagatat tagtctttct gataatacta cacctgtaaa attgaatgct caaactgaga
    10741 tttctgaaca aacagcagct ggggaactag atggaggaaa tgatgtatct gatctacact
    10801 catctgaaga aacgaatacc aaaatgaaaa attatgaaga aatgatgatc ggcgaggcaa
    10861 tggctgaaac tggccatgat ggtgaaacag agaatgaggg cataactacc aaaacctcaa
    10921 agcctgatga agctgaaaca aacatgttga ctgcagaaat ggacaacttt gtttgtgaca
    10981 cagttgaaat gagcactgaa gaaggaatca ttgacgctaa taaaactgaa acaaatactg
    11041 agtatagtaa atctgaagaa aaattagata acaatcaaat ggtaatggaa agtgatattt
    11101 tacaggaaga tcaccatact tcacagaaag tggaggaacc atcacagtgt ctggcatctg
    11161 gaacagctat ctctgagcta ataatagaag acaataatgc atctcctcaa aaactaaggg
    11221 aacttgatcc ttcacttgtg tcagcaaatg acagtcctag tggcatgcag acacgctgtg
    11281 tctggtctcc tttggcttct ccgtctacga gcattttaaa gagaggacta aaaagatccc
    11341 aagaagatga aatctcatca cctgttaata aggttcgccg tgtctccttt gcagatccaa
    11401 tataccaagc aggattggca gatgacattg atagacggtg ctctattgtt aggtcccatt
    11461 cttccaatag ttctcccata ggaaaaagtg ttaaaacttc tcctactaca caatctaagc
    11521 ataataccac ttcagccaaa ggatttctgt ccccaggatc acgtagccct aaatttaaga
    11581 gctcaaagaa gtgtttaatt tcagaaatgg ccaaagaatc cataccatgc ccaacagaaa
    11641 gtgtttaccc accattggtg aactgtgtgg caccagttga catcatttta cctcagatta
    11701 catcaaacat gtgggcaaga ggcctgggac aactcattag agctaagaat ataaaaacta
    11761 ttggtgattt gagtactctt acagcatctg aaataaaaac tcttcctatc cgttctccaa
    11821 aagtgtccaa tgtaaaaaag gctctcagaa tatatcatga gcagcaggtg aagactcgtg
    11881 gactagaaga gattccagtt tttgatattt ctgaaaaaac agtaaatgga atagaaaata
    11941 aatctttgtc acctgatgaa gaaagacttg tctcagatat aattgatcct gttgctttag
    12001 aaattccatt atccaaaaac cttgtggcac agattagtgc tcttgctctt cagctggatt
    12061 cagaagatct tcataattat tcaggaagcc aactatttga aatgcacgag aaactaagtt
    12121 gtatggcaaa ctctgtaata aaaaatctac agtcacgttg gagatcacca tcccatgaaa
    12181 attctattta gctcgagcgg gatcaattcc gccccccccc taacgttact ggccgaagcc
    12241 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
    12301 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
    12361 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
    12421 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
    12481 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
    12541 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
    12601 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
    12661 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
    12721 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatgaaa
    12781 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
    12841 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
    12901 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
    12961 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
    13021 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
    13081 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
    13141 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
    13201 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
    13261 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
    13321 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
    13381 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
    13441 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
    13501 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
    13561 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
    13621 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
    13681 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
    13741 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
    13801 gtcgagctca agcttcgaat tcgataatca acctctggat tacaaaattt gtgaaagatt
    13861 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
    13921 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
    13981 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
    14041 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
    14101 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
    14161 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
    14221 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    14281 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
    14341 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
    14401 ggccgcctcc ccgcatcggg aattcgagct cggtaccttt aagaccaatg acttacaagg
    14461 cagc
//
