LOCUS       sequence-105709-        4019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of synthetic human USP7 tagged with GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Faesen AC, Dirac AM, Shanmugham A, Ovaa H, Perrakis A, Sixma TK
  TITLE     Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like 
            domain and allosteric regulation by GMP-synthetase.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):147-59. doi: 
            10.1016/j.molcel.2011.06.034.
  PUBMED    21981925
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2011.06"; journalName: "Mol Cell"; date: 
            "2011-10-7- 7"; volume: "44"; issue: "1"; pages: "147-59"
COMMENT     Sequence Label: pFastbac1-GST-hUSP7
FEATURES             Location/Qualifiers
     source          1..4019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..660
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     618..640
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             667..690
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggatccatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
       61 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      121 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      181 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      241 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      301 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      361 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      421 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      481 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      541 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      601 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      661 tcggatctgg aagttctgtt ccaggggcca actagtacta gtatgaacca ccagcaacag
      721 cagcagcaac agaaagcagg tgaacagcaa ctctctgaac cggaagatat ggaaatggaa
      781 gcaggtgata ccgacgatcc accgcgtatt acccagaacc cggttattaa cggtaacgtt
      841 gcattaagcg atggtcacaa caccgcagaa gaagacatgg aagatgacac tagctggcgt
      901 agcgaagcaa ccttccagtt caccgttgaa cgtttctctc gtctgagcga aagcgttctg
      961 tctccaccgt gcttcgttcg taacttaccg tggaaaatca tggttatgcc gcgtttctat
     1021 ccggatcgtc cgcatcagaa aagcgttggc ttcttcctgc agtgcaacgc agaaagcgat
     1081 agcacctcct ggagctgcca cgcacaggca gttctgaaaa tcattaacta ccgtgatgat
     1141 gaaaagagct tctctcgtcg tattagccac ttattctttc acaaagagaa cgattggggt
     1201 ttcagcaact tcatggcatg gtctgaagtg actgatcctg aaaagggttt catcgacgat
     1261 gacaaagtga ctttcgaagt gttcgttcag gcagatgcac cacatggtgt agcttgggac
     1321 agcaagaaac acactggcta tgtaggttta aagaaccagg gtgcaacctg ctacatgaac
     1381 tctctgctcc agaccctgtt ctttaccaac cagcttcgta aagcagttta catgatgcct
     1441 acggaaggtg atgacagctc caaatctgta ccacttgctc tgcagcgtgt gttctacgag
     1501 ttacagcact ctgataaacc agttggtacc aaaaaactga ccaaatcctt cggttgggaa
     1561 accctggaca gcttcatgca gcatgatgtt caagaactgt gtcgtgtact cctggataac
     1621 gttgagaaca agatgaaagg cacgtgcgtg gaaggcacca ttccgaaatt attccgtggc
     1681 aaaatggtaa gctacattca gtgcaaagaa gttgactacc gtagcgatcg tcgcgaagac
     1741 tactatgaca ttcagctctc catcaaaggt aagaaaaaca tcttcgaatc cttcgttgac
     1801 tacgttgcag ttgaacagtt agatggtgac aacaaatacg atgctggcga acatggtctg
     1861 caggaagcag aaaaaggtgt gaagttcctg actctgcctc cggttcttca tctgcaactg
     1921 atgcgcttca tgtatgatcc gcagaccgat cagaacatca aaatcaacga tcgtttcgaa
     1981 tttcctgaac agttaccact ggatgagttc ttgcagaaaa ctgatccgaa agatcctgcg
     2041 aactacattc tgcatgctgt tctggttcac agcggtgaca atcatggcgg tcattatgtt
     2101 gtgtacttaa accctaaagg tgatggcaaa tggtgcaaat tcgatgacga tgttgtgtct
     2161 cgctgcacca aagaagaagc tattgaacac aactatggtg gtcacgacga tgacctgtct
     2221 gtacgtcact gcaccaacgc atatatgtta gtgtacattc gtgaaagcaa actgagcgaa
     2281 gttctgcagg cagttaccga tcacgacatt ccacagcagc tcgttgaacg tctgcaggaa
     2341 gagaaacgca ttgaagctca gaaacgcaaa gaacgtcagg aagcacactt atacatgcag
     2401 gttcagatcg tggctgaaga tcagttctgt ggtcatcagg gcaacgatat gtacgatgaa
     2461 gagaaagtta aatacaccgt gttcaaagtt ctgaaaaact cctctcttgc agaatttgta
     2521 cagtccttaa gccagacgat gggcttccct caggaccaga ttcgtctgtg gccgatgcag
     2581 gcacgtagca acggtaccaa acgtccagcg atgctggaca acgaagcaga tggcaacaaa
     2641 accatgattg aactcagcga caatgaaaac ccttggacga tcttcttaga aactgttgat
     2701 ccggaactgg cagcgtctgg tgcgactctt ccgaaattcg acaaagacca cgacgttatg
     2761 ctgttcctga aaatgtacga tccgaaaact cgctcgttga actactgtgg tcacatctac
     2821 actccgatct cctgcaaaat tcgtgacctg ttaccagtga tgtgtgatcg tgctggcttc
     2881 attcaggaca cctctctgat tctctacgaa gaagtgaaac cgaacctgac cgaacgcatc
     2941 caggactacg atgtgtctct ggacaaagcg ttagatgagt tgatggatgg tgacatcatt
     3001 gtgttccaga aagacgatcc tgaaaacgac aactctgaac ttccgactgc taaagaatac
     3061 tttcgtgatc tgtatcaccg tgtagatgtg atcttctgcg acaaaaccat tccgaacgat
     3121 ccaggctttg tggtaacctt atccaatcgc atgaactact ttcaggttgc gaaaactgtt
     3181 gctcagcgtc tgaacactga tccgatgctg cttcagttct tcaaatctca aggctatcgt
     3241 gatggtcctg gcaatccttt acgtcacaac tatgaaggca ctctgcgtga tttgctgcag
     3301 ttcttcaaac ctcgtcagcc aaagaaactg tactatcagc aactgaagat gaaaatcacc
     3361 gacttcgaaa accgtcgcag cttcaaatgc atctggttaa actctcagtt tcgcgaagaa
     3421 gagatcactc tgtacccaga caaacatggc tgtgttcgtg atctgctcga agaatgcaag
     3481 aaagctgtag aacttggcga aaaagcgtct ggcaaacttc gtctgctcga aattgtgagc
     3541 tacaaaatca tcggtgtgca tcaagaagat gaactgttag aatgtctgtc tccagcgacc
     3601 agccgtacct ttcgtattga agaaattcct cttgatcagg ttgacattga caaagaaaac
     3661 gaaatgctgg ttaccgttgc acacttccac aaagaagtgt ttggtacctt cggtattccg
     3721 tttctgttac gcattcatca aggtgaacac tttcgtgaag tgatgaaacg cattcagtct
     3781 ctgctggaca tccaggagaa agagttcgaa aagttcaaat ttgctattgt tatgactggt
     3841 cgtcatcagt acatcaacga agatgaatat gaagtgaact taaaagactt cgaacctcag
     3901 ccaggcaaca tgagccatcc acgtccgtgg cttggtctgg accacttcaa caaagctccg
     3961 aaacgctctc gttacaccta cttagaaaaa gcgatcaaaa ttcacaacta agcggccgc
//
