LOCUS       sequence-105719-       13163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for the Col1a1 locus that contains a Neo resistance
            cassette flanked by FRT sites, followed by an ATG-less Hygromycine 
            resistance gene. The homology arms match the sequence of FVB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Huijbers IJ, Del Bravo J, Bin Ali R, Pritchard C, Braumuller TM, van
            Miltenburg MH, Henneman L, Michalak EM, Berns A, Jonkers J
  TITLE     Using the GEMM-ESC strategy to study gene function in mouse models.
  JOURNAL   Nat Protoc. 2015 Nov;10(11):1755-85. doi: 10.1038/nprot.2015.114. 
            Epub 2015 Oct 15.
  PUBMED    26492136
REFERENCE   2  (bases 1 to 13163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2015.114"; journalName: "Nat Protoc"; date: 
            "2015-11"; volume: "10"; issue: "11"; pages: "1755-85"
COMMENT     Sequence Label: pCol1a1-frt (FVB)
FEATURES             Location/Qualifiers
     source          1..13163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1944..1949,1950..1967,1968..1974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1987..2009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2006..2022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2041..2061
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2043..2061
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    5382..5429
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5440..5939
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5475..5498)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5872..5891
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5953..6756
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6016..6035)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6626..6645
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6766..7227
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    7375..7509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7629..7763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7809..7826)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7815..8039
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8323..8370
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8378..9400
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8378..9400
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9530..9651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12391..12410)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12392..12410)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12420..12437)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12420..12436)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12429..12451)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(12577..13032)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12714..12735)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13059..13163
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2041 gcaattaacc ctcactaaag ggaacaaaag ctgggtacgg gccggccctc gaggtcgcac
     2101 tggtgacagc ggccctgctg tatgtagccc cacatcctct gcctcatgac cctcccccaa
     2161 gaagacttga atacctggaa accttgatct ccttcctcta cacagggtcc ccccggccct
     2221 cctggacccc ctggccctcc tggacctccc agtggcggtt atgacttcag cttcctgcct
     2281 cagccacctc aagagaagtc tcaagatggt ggccgctact accgggccga tgatgctaac
     2341 gtggttcgtg accgtgacct tgaggtggac accaccctca agagcctgag tcagcagatt
     2401 gagaacatcc gcagccccga aggcagccgc aagaaccctg cccgcacatg ccgcgacctc
     2461 aagatgtgcc actctgactg gaagagcggt aaggattaac cccagccacc tccccccccc
     2521 cctttagcct accatgctgc ttcccccatg ttttcataca ttccactttc catatggtgc
     2581 tggctgccta ctcctcctga ctgctttgcc ttgcccactt caggagagta ctggatcgac
     2641 cctaaccaag gctgcaacct ggacgccatc aaggtctact gcaacatgga gacaggtcag
     2701 acctgtgtgt tccctactca gccgtctgtg cctcagaaga actggtacat cagcccgaac
     2761 cccaaggaaa agaagcacgt ctggtttgga gagagcatga ccgatggatt cccggtacgt
     2821 gagctgaacc ccagagcaac tctacaggag atgggttagc ctcttatcca gaaagcacag
     2881 gaagtcctgg agtggattta atctgcttag tggaagggca gatacagatc taggatacag
     2941 gagttctggg taggaaagcc agggacgaga aggtccacgt gatcgctcat tctctcctct
     3001 ctctggcccg tgcagttcga gtacggaagc gagggctccg accccgccga tgtcgctatc
     3061 cagctgacct tcctgcgcct aatgtccacc gaggcctccc agaacatcac ctatcactgc
     3121 aagaacagcg tagcctacat ggaccagcag actggcaacc tcaagaaggc cctgctcctc
     3181 cagggatcca acgagatcga gctcagaggc gaaggcaaca gtcgcttcac ctacagcacc
     3241 cttgtggacg gctgcacggt gagtccccca gactgggtcc agcctgtctc tttgggcatc
     3301 tggtttagcc ttctggcaga acagagtgag cctcacttca ttcctacctt ggtaatgacc
     3361 ctctcccgct gtcttcattc acaccagagt cacaccggaa cttggggcaa gacagtcatc
     3421 gaatacaaaa ccaccaagac ctcccgcctg cccatcatcg atgtggctcc cttggacatt
     3481 ggtgccccag accaggaatt cggactagac attggccctg cctgcttcgt gtaaactccc
     3541 tccaccccaa tctggttccc tcccacccag cccacttttc cccaaccctg gaaacagacg
     3601 aacaacccaa actcaatttc ccccaaaagc caaaaatatg ggagataatt tcacatggac
     3661 tttggaaaac attttttttc ctttgcattc acctttcaaa cttagttttt acctttgacc
     3721 aactgaacgt gaccaaaaac caaaagtgca ttcaacctta ccaaaaaaga aaaaaaaaaa
     3781 agaataaata aataactttt taaaaaagga agcttggtcc tcttgcttga agacctatgt
     3841 gggtataagt cccttcctgc ccacttggct tatgataccc taatgctgcc ttttctgctc
     3901 ctttctccac cccctcttgg ggcctctcct ccattgctcc ccaaatttaa gtctccccca
     3961 agacacagga aataatgcat tgtctgccca gcaaacaaag gcaatgctga aatgtcccac
     4021 cagcccctca accccgtcta cttccctacc cagcaccctc aaatcctgct gggacatggg
     4081 gttcttggac tgttgaagga acctaaccat ctggcatctc catggcctct gcaacaaacc
     4141 ccccactttt ttctctcccc ccccccccag ggagggcctg tgctttgggc agccacctgc
     4201 ccctctcagg ggtttggagc caggcagggt cacagcagac tggaaacatc ggacatgcat
     4261 gtgcaggctg ggtgggagag accgttctat tcctcagtgc aattgtgttg ctgaaagact
     4321 acctcgttct tgtctttgtg tgtcaccggg gcaactgtgt gggggcgggg atgggggcag
     4381 ggtggcagca cgcccagttt ggtatcaaag gtgctacatc tctgtgaagg ggtggggtgg
     4441 gaaggaattt ctggtgctat agaagctgag atgctcccta gaccagcaaa tgtttctttt
     4501 gttcaaagta ttttttattc tttttttttt tttttaatgg atagggactt gtgtgaattg
     4561 ttggggtttt tttttttttt tttggttttg tttttttttg ttttgttttg ttttttttcc
     4621 tgaaggtgct atttaacaag ggagaagaga gtgcggggac ttcaccctgc ccactctcta
     4681 ctctctctcc actcttctag ttcctgggcc tatctgatct ctctctttct tctgaaaccc
     4741 tcccctcttg ctgctgctcc ctcccctctg cctctctctt ggtctgtcct gcatcagggt
     4801 ttcagagcac cactttccaa agcacaaaac agtttttacc cctgggctgg gaggaaacaa
     4861 gagactctgt acctattttg tatgtgtata ataatttgag atgtttttaa ttattttgat
     4921 tgctggaata aagcatgtgg aaatgaccca atgcatgtgc agtggcctct gaatttcctt
     4981 cctgggacgt ggggaggtgg ggatccaggg acagggctct gggatggggg acttgccttc
     5041 tacccctgcc ctttctctct ccctcacttc tcatccagat attgccacct tcctctcttg
     5101 cctgtcttat gccttagacc ttttttcttg cctcttttac cttttcctct ttccagtctt
     5161 ccttgtgcat ccccagatcc ccagcttcac cagttcaatc atcccaggtg cacagcattg
     5221 cggacatgct cgcacgtact tcattcccag atctctctct ctctctctct ctctctctct
     5281 ctctcacaca cacacacaca cacacacaca cacacacaca tacacacaca cacacctcag
     5341 gcccgccatc ccaacaatac atcacatctc catgcggccg cgaagttcct attccgaagt
     5401 tcctattctc tagaaagtat aggaacttcc aattctaccg ggtaggggag gcgcttttcc
     5461 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     5521 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     5581 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     5641 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     5701 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     5761 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     5821 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     5881 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     5941 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     6001 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6061 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6121 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6181 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6241 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6301 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6361 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6421 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6481 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
     6541 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6601 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6661 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6721 atcgccttct atcgccttct tgacgagttc ttctgagggg atccgctgta agtctgcaga
     6781 aattgatgat ctattaaaca ataaagatgt ccactaaaat ggaagttttt cctgtcatac
     6841 tttgttaaga agggtgagaa cagagtacct acattttgaa tggaaggatt ggagctacgg
     6901 gggtgggggt ggggtgggat tagataaatg cctgctcttt actgaaggct ctttactatt
     6961 gctttatgat aatgtttcat agttggatat cataatttaa acaagcaaaa ccaaattaag
     7021 ggccagctca ttcctcccac tcatgatcta tagatctata gatctctcgt gggatcattg
     7081 tttttctctt gattcccact ttgtggttct aagtactgtg gtttccaaat gtgtcagttt
     7141 catagcctga agaacgagat cagcagcctc tgttccacat acacttcatt ctcagtattg
     7201 ttttgccaag ttctaattcc atcagaagct tgcagatctg cgactctaga ggatctgcga
     7261 ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     7321 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     7381 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7441 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7501 gtctggatct gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac
     7561 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     7621 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7681 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7741 atgtatctta tcatgtctgg atccttctag agtcgagtct agagggcccg tttaaacccg
     7801 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7861 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7921 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7981 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     8041 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     8101 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8161 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8221 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8281 cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag
     8341 ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac
     8401 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     8461 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     8521 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     8581 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     8641 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     8701 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     8761 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     8821 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     8881 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     8941 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     9001 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     9061 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     9121 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     9181 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     9241 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     9301 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     9361 aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga
     9421 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9481 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     9541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9661 tataccgtcg agacctgagg cctgggaggt gttgcccatg gatcctggga ggttcatgag
     9721 ccctcaaagg gattcaagca gcagaaacag ggactgagac atggaggacc aggttgctcc
     9781 ttggtcacac atggaggggt agctggcctc tccccacctc tagcccaaag aaaccataat
     9841 ttagtataga aggggccttc taatgctggg gtgtcacaag gaggatgaga atttcttggg
     9901 tcacccttga tctcaccaga gagaggtgcc cagccctcaa ggaactctcc ccgggggatc
     9961 ttagagagct gtggtggaac ttccggggtg tcaccagaaa ggacaggacc ccacatcaca
    10021 gaggtgcccg ggacgatagc atgctggctc ggtacaggga gcccgtggaa ttgttccccc
    10081 cttcttcctg cccccttctt tccttttcct ttatccccaa aactttttgc tttttcttct
    10141 tccttttccc tctctccctc tgtccatctg agaacctgag gccctagagg gatggtaact
    10201 aattgtcccc cacatctcag agaatgggga cggtgacata gtagaaggcc cgagaatcca
    10261 gcaggcagaa gtctggggtg aaccagacag tagacataag gacacagatg acccctttca
    10321 ctcggggaga caggtctcaa atttgaaagg aaggaacagg ccatttgtag cttccctctc
    10381 ttgtgctacc agtctactgt ggcacccata ggacacgttc atatggtcac gaagtatcca
    10441 agactattaa tgatggcctt taataattta ttattagcac tttgcatgag gacaacgtga
    10501 aaggaaaatt tatctagaga ggaaaagaag ttagggaatg taaagatcta ttcagacaca
    10561 aattatccaa actcctgtag tactgataga cccactgatt gacacgttga ttgggtaaaa
    10621 cctttcttcc caggcatcac cagtcttggg actcttcctg gctagaccct atctcctccc
    10681 atcctcacag ggtccatcct tctgaactca gcatccgagc tgtacctggc cactgctcgc
    10741 ttgtctgaac ttactgtctc ctccacgagc ccccatctgt cataccagac aaagggtgtg
    10801 actgtaattt ctacacatat aaccctcctt ctcattttat ttccctccgt attcctggca
    10861 taatgtcccc tctctctact gactaatccc tcatcctcca agggcaggtt tcctcccctc
    10921 ttccctaacc aagccctctg aggtcccaac acaccatcag cccattagcc ggtatgttat
    10981 taccaggctc ccgtggtcat ggtccactat gttcttttca ggcctgacaa ttctgtgcag
    11041 ggctgggcac agtctccata ggtaccaggg agaggcaaga tggtgagaga ggtcctccgg
    11101 aggatataag tacagagctg tggaaaggtg agggtgaagg tgagaggaag aacaaacaaa
    11161 ctagccaaga aagggagcaa atgacagctt cctggcgtga caggtagtca agcacttgaa
    11221 atgtgtctcc ccgtgataaa ggacagtagt cctaacttgt actactgcaa tcattaatta
    11281 attaattaat taattaatag ctacaaatgg gctgaaggaa acagaactcc agacaaagga
    11341 gcgaataact tctcactgtg cgcctgaaaa tctatacata tacatacatg tacacacgtg
    11401 gatatacaca agggcatgtg caccttgtga taggccttgt ttgcagcacc ccgcagcggc
    11461 tgtcacgtgg catgggctgg tatgtgctct aaatgtgtgt atagaacagg gacggggaca
    11521 acaaagagat cattgtatca cactctgaac ccaagccctc ctttccgctg acatcattgc
    11581 tgccttaaat acagatgcca ggccctgttt ccaagaccct cactgtcccc tgtgtgctaa
    11641 cacagcacct actcccagct ctgctctatg acagtcacct tcgttttttg tggggaagag
    11701 tagtctcttg gagctgatac ttcagtgaaa tccaagtgga aaatctacag ggaaagaaga
    11761 cttcattttg actttttttc tctctcttgc tgtccttttg gaatccaagt ccctttgggg
    11821 agggacctgg agcatggtca tctcctggat ggatttgctg taacctaaag atggtgggtt
    11881 ttccccttta ttcagctttt caatcctgtt tctgtttcta gctgtctcca aaactcccat
    11941 agacagagag ttgcatgtga ccggagagga aaggcttcta gctggagctt ggggacctta
    12001 gcggtgtgag ggaggacaag gggagtaccc acccatgagg ggaggcactc attaaggcac
    12061 aggaaggcct gggtggtaac gtgagggagt gaatgggagc tgtgggtggc cccgtgtggg
    12121 tgtcacagtg gggacctgag tgaatgtgtc tctttctgtg cccacttacc tgtaagcaaa
    12181 gacatctatg tccacaggcc ccttccggtt tggtgtggca taggtgggag atcacagcct
    12241 ctggacatac aagtcttaca agtttatccg tgcaggctga tgggttaaag ctgagcagag
    12301 agacacttgg gaaaaccagg aggggctggt gtggaagaag acaagcatcc caaagtcccc
    12361 cgcactagtg gcgcgcccag ctccaattcg ccctatagtg agtcgtatta cgcgcgctca
    12421 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    12481 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    12541 ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt
    12601 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    12661 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    12721 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    12781 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    12841 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    12901 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    12961 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    13021 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    13081 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    13141 aataatattg aaaaaggaag agt
//
