LOCUS       sequence-105742-         415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector endoding for mOrange and mouse CTCF with a short 
            biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Nakahashi H, Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, 
            Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, 
            Pugh BF, Lobanenkov V, Hager GL, Casellas R
  TITLE     A genome-wide map of CTCF multivalency redefines the CTCF code.
  JOURNAL   Cell Rep. 2013 May 30;3(5):1678-89. doi: 
            10.1016/j.celrep.2013.04.024. Epub 2013 May 23.
  PUBMED    23707059
REFERENCE   2  (bases 1 to 415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.04.024"; journalName: "Cell Rep"; date: 
            "2013-05-30- 30"; volume: "3"; issue: "5"; pages: "1678-89"
COMMENT     Sequence Label: pMy-MouseCTCFbiotag-T2A-mOrange
FEATURES             Location/Qualifiers
     source          1..415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..87)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(106..150)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 ggccatgtta ttctcctcgc ccttgctcac catagggccg ggattctcct ccacgtcacc
       61 gcatgttaga agacttcctc tgccctccct cgagctcggc gcgccttcgt gccattcgat
      121 tttctgagcc tcgaagatgt cgttcaggcc ggaccggtcc atcatgctga ggatcatctc
      181 aggcgtgagg tctccgttgg gggcgtctgt ggtggctgcc tgagcctcct cttcttcccc
      241 ctccgcaggc tcggcatctg gctccttttt gacttcaact ataatgttct caattgcacc
      301 tgtattctga tcttcgacct gaatgatggc tgttggctgg ttctgtttgg gctggttggt
      361 tcttccagga ggtcgccccc ttctcttctt ggcaggtggg ggggccgggg ccaca
//
