LOCUS       sequence-105773-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pET28A-p53 (1-73) (P12A P13A)
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             322..339
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(392..410)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      406..453
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(577..596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     787..808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgatggagg agccgcagtc agatcctagc
      121 gtcgaggccg ctctgagtca ggaaacattt tcagacctat ggaaactact tcctgaaaac
      181 aacgttctgt cccccttgcc gtcccaagca atggatgatt tgatgctgtc cccggacgat
      241 attgaacaat ggttcactga agacccaggt ccagatgaag ctcccagaat gccagaggct
      301 gctccccgcg tgtgactcga gcaccaccac caccaccact gagatccggc tgctaacaaa
      361 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      421 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
      481 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      541 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      601 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      661 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      721 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      781 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
      841 ttg
//
