LOCUS       sequence-105774-         380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector encoding for GFP and the biotinylating enzyme BirA
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Nakahashi H, Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, 
            Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, 
            Pugh BF, Lobanenkov V, Hager GL, Casellas R
  TITLE     A genome-wide map of CTCF multivalency redefines the CTCF code.
  JOURNAL   Cell Rep. 2013 May 30;3(5):1678-89. doi: 
            10.1016/j.celrep.2013.04.024. Epub 2013 May 23.
  PUBMED    23707059
REFERENCE   2  (bases 1 to 380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.04.024"; journalName: "Cell Rep"; date: 
            "2013-05-30- 30"; volume: "3"; issue: "5"; pages: "1678-89"
COMMENT     Sequence Label: pMy-BirA-T2A-eGFP
FEATURES             Location/Qualifiers
     source          1..380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..224
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(270..323)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
       61 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      121 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      181 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      241 gtgaacagct cctcgccctt gctcaccata gggccgggat tctcctccac gtcaccgcat
      301 gttagaagac ttcctctgcc ctctttttct gcactacgca aggatatttc accgcccatc
      361 cagggtttta ttattccatc
//
