LOCUS       sequence-105787-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of A5NT sgRNA from the arabinose-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PBAD-sgRNA-A5NT
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        50..125
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             146..175
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             191..208
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(264..281)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      434..480
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(533..550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata taataccctc cgagtgtttg ttttagagct
       61 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      121 ggtgcttttt ttgaagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag
      181 cgccgtcgac catcatcatc atcatcattg agtttaaacg gtctccagct tggctgtttt
      241 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
      301 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
      361 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
      421 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      481 ctgttgtttg tcggtgaact ggatccttac tcgagtctag actgcaggct tcctcgctca
      541 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      601 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      661 agcaaaaggc caggaaccgt aaaaaggccg cgttgct
//
