LOCUS       sequence-105795-         559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of GFP (CAG promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAG-GFP
FEATURES             Location/Qualifiers
     source          1..559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(104..123)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(185..207)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc
       61 ggtaccgtcg actgcagaat tctttgccaa aatgatgaga cagcacaata accagcacgt
      121 tgcccaggag ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc
      181 cgccggtcac acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt
      241 ccccccgcgg acagccccga ggctggagag ggagaagggg acggcggcgc ggcgacgcac
      301 gaaggccctc cccgcccatt tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc
      361 tagagggggt gcggcggcgc ctcccagatt tcggctccgc acagatttgg gacaaaggaa
      421 gtccctgcgc cctctcgcac gattaccata aaaggcaatg gctgcggctc gccgcgcctc
      481 gacagccgcc ggcgctccgg gggccgccgc gcccctcccc cgagccctcc ccggcccgag
      541 gcggccccgc cccgcccgg
//
