LOCUS       sequence-105815-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-mCherry expressed under 
            MtBCP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-ACTLr
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..93)
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     protein_bind    complement(96..120)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttgatgatg gccatgttat cctcctcgcc cttgctcacc atttcttcct ttgagatgct
       61 ttcgaatttc ttgatcaaat ctgcgacacc catgaagcct gcttttttgt acaaacttgc
      121 ttgatcaaaa tttgattttg gaaaatattt ctctctaaat gtagaaatta actttaaaaa
      181 aatgagtgtc aaaattttat ttcctagaat aaaatctcaa tcaaaggaat agcaagttag
      241 caaatgtaat tgttttgatt tgatttgatg aattgtgcat atcaacttag gctatatata
      301 gttgtcatgt gtgggtgtat tgattttatc gtcacaacaa taatagaata tttgtttgct
      361 attataaacg gctaccggat ttggtcggct ttttactaag acgaggttga atttccacaa
      421 cttattattt ttttttttac ctttttggaa tacacttata ttattttata ttaatgtaaa
      481 gaaactttct atcacttgaa caaaaagaaa gttttctttt gtttttattt aaccacgtgg
      541 acatacttgt ttttatttgg cagtaaacaa gtatgcaata tagtgtggac cgtgtcaaag
      601 ggaaatagaa acagtatata tagttcctct tccgggcttc gttataattg agttgtctcc
      661 atgcagaaag tttacacata aataatgttt gtttcttacg agtttcttta tttggaaaat
      721 tagagatgta atcgagtatt gacacttgga gtacaaatac ttgaggagtc tcgaatatat
      781 caagttctca agcttaaatt agagatgtaa tcgagtattg accttgtttt
//
