LOCUS       sequence-105820-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mRNA synthesis or expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Matoba S, Liu Y, Lu F, Iwabuchi KA, Shen L, Inoue A, Zhang Y
  TITLE     Embryonic Development following Somatic Cell Nuclear Transfer 
            Impeded by Persisting Histone Methylation.
  JOURNAL   Cell. 2014 Nov 6;159(4):884-95. doi: 10.1016/j.cell.2014.09.055. 
            Epub 2014 Oct 30.
  PUBMED    25417163
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.055"; journalName: "Cell"; date: "2014-11-6-
            6"; volume: "159"; issue: "4"; pages: "884-95"
COMMENT     Sequence Label: pcDNA-flag-mKdm4d-polyA
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(99..118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        290..669
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        670..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     824..844
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     918..937
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        918..936
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             966..989
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
       61 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
      121 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      181 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      241 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      301 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      361 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      421 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      481 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      541 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      601 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      661 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      721 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      781 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      841 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      901 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagttaagc
      961 ttatggacta caaagacgat gacgacaagg g
//
