LOCUS       sequence-105829-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Cyclin B1 with R42A mutation in destruction 
            box to make cyclin B1 non-destructable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Clute P, Pines J
  TITLE     Temporal and spatial control of cyclin B1 destruction in metaphase.
  JOURNAL   Nat Cell Biol. 1999 Jun;1(2):82-7.
  PUBMED    10559878
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "1999-06"; volume: "1(2)"; pages: "82-7"
COMMENT     Sequence Label: (R42A) Cyclin B1-GFP (62)
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     390..418
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgttgtggga gttgtagttg tattccaact tgtggccgag gatgtttccg tcctccttga
       61 aatcgattcc cttaagctcg atcctgttga cgagggtgtc tccctcaaac ttgacttcag
      121 cacgtgtctt gtagttcccg tcgtccttga agaagatggt cctctcctgc acgtatccct
      181 caggcatggc gctcttgaag aagtcgtgcc gcttcatatg atctggatat cttgaaaagc
      241 attgaacacc ataagtgaga gtagtgacaa gtgttggcca tggaacaggt agttttccag
      301 tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca ccctctccac
      361 tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt gggacaactc
      421 cagtgaaaag ttcttctcct ttactgaatt gcgctcctgc cacctttgcc acagccttgg
      481 ctaaatcttg aactagtgca gaattcagct gtggtagagt gctgatctta gcatgcttcg
      541 atgtggcata cttgttcttg acagtcatgt gctttgtaag tccttgattt accatgacta
      601 cattcttagc caggtgctgc ataactggaa gaagagattc ttcagtatat gacaggtaat
      661 gttgtagagt tggtgtccat tcaccattat ccagaatttt cagtgctaag caaaaagctc
      721 ctgctgcaat ttgagaagga ggaaagtgca ccatgtcata gtccaacata gttagttcca
      781 tcaggtattt ggccaaagta tgttgctcga catcaacctc tccaatctta gatgctctcc
      841 gaaggaagtg caaaggtaga ggccgaccca gaccaaagtt taaagctctt agaatcttca
      901 tttccatctg tctgatttgg tgcttagtat aagtgttgtc agtcacaaaa gcaaagtcac
      961 caatttctgg agggtacatt tcttcatatt tgcttg
//
