LOCUS       sequence-105831-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QUAS driving GFP with beta crystalline CFP eye marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons 
            MJ, Halpern ME
  TITLE     Adoption of the Q transcriptional regulatory system for zebrafish 
            transgenesis.
  JOURNAL   Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. 
            Epub 2013 Jun 20.
  PUBMED    23792917
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2013.06.012"; journalName: "Methods"; date: 
            "2014-04-1- 1"; volume: "66"; issue: "3"; pages: "433-40"
COMMENT     Sequence Label: QUAS:GFP;betacrystallin:CFP
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..76
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    727..751
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(855..876)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgcgcgttc caatgcaccg ttcccggcaa cctagcatgc agataacttc gtataaagta
       61 tcctatacga agttatggca acgcgtttaa actacagctg tgctgggaag gctggctcgc
      121 tctggccttc ctcaccactg ccgcagcttt tatacgctgg caagggcagc acagcggcgc
      181 gcggcggaca atagaaacac gccgtcatca gagttcagcc gtgccgaagc gcagggtcat
      241 cgcatcgccc agaaggccgt aagcatttta ctgtcaatgg aaagccctga cggcggcact
      301 ttccctctca gcccgagctc tatgctatag gctgtatacc tatggctaag agaggtctgt
      361 gttcaggtca gaggtcacgt gatgataatc cgagcagagg agtcgctcgc tgatacactc
      421 cagagcagag tagatttcat actgaagtta ataatgtagc atgacatata ttagatggtg
      481 tgtgtgtgtg tgtgtatata catatatata tatatatggt agcgtattta gatggaattt
      541 attgatgtat tatttattat atgtatatac agatgaagtc aaaatttttc gctccctgta
      601 tattttcccc ttaaatcctg tttaagagaa agaaacatat ttctaatcat aatagtttta
      661 ataactcatt tctaataact gatttcttct ctctttgtca tgatgatagc accaattgct
      721 cgacggtcca ctttgtacaa gaaagctggg tgctataggg ctgcagaatc tagaggctcg
      781 agaggccttg aattcccaga tcttccaccg cccttgtaca gctcgtccat gccgagagtg
      841 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      901 ttgctcaggg cggactgggt gctcaggtag tgg
//
