LOCUS       sequence-105836-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL
            expressed under AtUBQ10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMU-ERr
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(270..294)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(525..545)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(553..570)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(553..569)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(562..584)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    658..682
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggagg agctgtacaa
      241 gagatctcat gacgagctgt aactgcagga cccagctttc ttgtacaaag tggaataagc
      301 ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt
      361 tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt
      421 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
      481 ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacgcaactt tattatacat
      541 agttgataat tcactggccg tcgttttaca acgtcgtgac tgggaaaacg agctcaagct
      601 tagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
      661 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
      721 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgca
//
