LOCUS       sequence-105837-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of A4T sgRNA from the arabinose-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-PBAD-sgRNA-A4T
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        52..127
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             148..177
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             193..210
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(266..283)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      436..482
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(535..552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(786..805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttatcgca actctctact gtttctccat atttacacct ccacaactag cgttttagag
       61 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      121 tcggtgcttt ttttgaagct tgggcccgaa caaaaactca tctcagaaga ggatctgaat
      181 agcgccgtcg accatcatca tcatcatcat tgagtttaaa cggtctccag cttggctgtt
      241 ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
      301 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
      361 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
      421 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      481 atctgttgtt tgtcggtgaa ctggatcctt actcgagtct agactgcagg cttcctcgct
      541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      661 ccagcaaaag gccaggaacc gtaaaaaggc tgcgttgctg gcgtttttcc acaggctccg
      721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      841 cctgccgctt accggatacc tgtccgcctt tctcccttcg g
//
