LOCUS       sequence-105844-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  synthetic circuit: AND gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Nielsen AA, Voigt CA
  TITLE     Multi-input CRISPR/Cas genetic circuits that interface host 
            regulatory networks.
  JOURNAL   Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
  PUBMED    25422271
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-11-24"; volume: "10:763. doi"; pages: " 
            10.15252/msb.20145735"
COMMENT     Sequence Label: pAN-AND
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        50..125
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             146..175
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(261..278)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      431..477
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tttatcgcaa ctctctactg tttctccata taatacccgt gtgacccgtg ttttagagct
       61 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      121 ggtgcttttt ttgaagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag
      181 cgccgtcgac catcatcatc atcattgagt ttaaacggtc tccagcttgg ctgttttggc
      241 ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
      301 aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
      361 gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
      421 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      481 ttgtttgtcg gtgaactgga tccttactcg agtctagact gcaggcttcc tcgataggaa
      541 gcaggaatag tttttacacc aacgggtcac acgggtatta tgctagcata atacctagga
      601 ctgagctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgg cttcaacttg
      661 aaaaagtggc accgagtcgg tgcttttttt ggaaacacag aaaaaagccc gcacctgaca
      721 gtgcgggctt tttttttcga ccaaagggct cactgactcg ctgcgctcgg tcgttcggct
      781 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatc
//
