LOCUS       sequence-105850-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Fujii H, Fujita T
  TITLE     An enChIP system for the analysis of genome functions in budding 
            yeast.
  JOURNAL   Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 
            10.1093/biomethods/bpac025. eCollection 2022.
  PUBMED    36325175
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Methods Protoc."; date: "2022-10-17"; volume: "7(1):bpac025. doi"; 
            pages: " 10.1093/biomethods/bpac025. eCollection 2022"
COMMENT     Sequence Label: 3xFLAG-dCas9/pTEF1p-CYC1t
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    61..564
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     605..623
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(661..683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     783..802
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtccttttc
       61 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
      121 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
      181 actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc tctcaggtat
      241 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
      301 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
      361 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
      421 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
      481 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
      541 cgtgtaagtt acaggcaagc gatccgtcta agaaaccatt attatcatga cattaaccta
      601 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
      661 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
      721 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      781 tgcggcatca gagcagattg tactgagagt gcaccataga tcacgacatt actatatata
      841 taatatagga agcatttaat agaacagcat cgtaatatat gtgtactttg cagttatgac
      901 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
      961 atcttgatcc ggagcttttc tttt
//
