LOCUS       sequence-105851-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Fujii H, Fujita T
  TITLE     An enChIP system for the analysis of genome functions in budding 
            yeast.
  JOURNAL   Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 
            10.1093/biomethods/bpac025. eCollection 2022.
  PUBMED    36325175
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Methods Protoc."; date: "2022-10-17"; volume: "7(1):bpac025. doi"; 
            pages: " 10.1093/biomethods/bpac025. eCollection 2022"
COMMENT     Sequence Label: 3xFLAG-dCas9/pTEF1p-CYC1t
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(226..900)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
       61 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
      121 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagatcg
      181 gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt
      241 tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct
      301 tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag
      361 tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa cccagtcaga
      421 aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat
      481 aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac
      541 gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc
      601 atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg
      661 ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt
      721 tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac
      781 acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg cctctgtgct
      841 ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat
      901 actccaagc
//
