LOCUS       sequence-105852-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNA-Guided, Nuclease-Inactive 
            VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation 
            of Endogenous Genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Chakraborty S, Ji H, Kabadi AM, Gersbach CA, Christoforou N, Leong 
            KW
  TITLE     A CRISPR/Cas9-Based System for Reprogramming Cell Lineage 
            Specification.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):940-7. doi: 
            10.1016/j.stemcr.2014.09.013. Epub 2014 Oct 23.
  PUBMED    25448066
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.09.013"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "940-7"
COMMENT     Sequence Label: TetO-FUW-VdC9BV
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(355..378)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(385..534)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     regulatory      549..558
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(640..664)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(661..699)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    732..750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    774..792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    816..834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    858..876
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    900..918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    942..960
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ttcctcgagt ctgtggaaaa aactatcatc gactttagcc atttcgttac taaagatttc
       61 ctgcaagtag catatcctgt tcttccgtcg tgtgtatctt cttcgagcgg tccgtttcag
      121 ccgagtagct tctgcggtct cgccgctgtc aaacagaagg gcgccgatga gattcttttt
      181 gatggagtgg cggtcggtat taccgagaac cttgaatttc ttgcttggca ccttatactc
      241 atcggttatg acagcccagc caacagagtt tgtgccaatg gcgagtccga tagagtattt
      301 cttatccata gagccaccgc ccccgctgcc tccaccgccg cttcctccac ctcccttatc
      361 gtcatcgtcc ttatagtcgt tgatgagcat atccaggtca aagtcgtcca gcgcgtctga
      421 tcccagcatg tccagatcga agtcatccaa tgcatctgag cccagcatat ccaggtcgaa
      481 gtcgtcgagg gcgtcgctac ccaacatatc gagatcgaaa tcgtcaaggg cgtcggctct
      541 cccactgccc atggtggcta gcaattcggg gccgcggagg ctggatcggt cccggtgtct
      601 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
      661 agctctgctt atataggcct cccaccgtac acgcctacct cgacccgggt accgagctcg
      721 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      781 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
      841 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
      901 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
      961 gtggtaaact cgactttcac ttttctctat cactga
//
