LOCUS       sequence-105867-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of nuclear envelope transmembrane protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Malik P, Zuleger N, de las Heras JI, Saiz-Ros N, Makarov AA, Lazou 
            V, Meinke P, Waterfall M, Kelly DA, Schirmer EC
  TITLE     NET23/STING promotes chromatin compaction from the nuclear envelope.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e111851. doi: 
            10.1371/journal.pone.0111851. eCollection 2014.
  PUBMED    25386906
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e111851. doi"; pages: " 
            10.1371/journal.pone.0111851. eCollection 2014"
COMMENT     Sequence Label: NET31 mRFP-N2 (490)
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(136..804)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVIQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     primer_bind     complement(195..218)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     662..680
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcta gagtcgcggc
      121 cgcttacttg tacagggcgc cggtggagtg gcggccctcg gcgcgctcgt actgttccac
      181 gatggtgtag tcctcgttgt gggaggtgat gtccagcttg atgtcggtct tgtaggcgcc
      241 gggcagctgc acgggcttct tggccatgta ggtggtcttg acctcggcgt cgtagtggcc
      301 gccgtccttc agcttcagcc tcatcttgat ctcgcccttc agggcgccgt cctcggggta
      361 catccgctcg gtggaggcct cccagcccat cgtcttcttc tgaattacgg ggccgtcgga
      421 ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga
      481 gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa
      541 gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc
      601 cttggagccg tactggaact gaggggacag gatgtcccag gcgaagggca gggggccgcc
      661 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
      721 ctcgatctcg aactcgtggc cgttcacgga gccctccatg cgcaccttga agcgcatgaa
      781 ctccttgatg acgtcctcgg aggaacggcc catggccatt cggatcccgg gccgctcgcc
      841 aaagatccac aatatattca cacca
//
