LOCUS       sequence-105869-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used with pSpec  to generate an AND gate without memory .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pAND_yfp
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    77..95
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    102..120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgatgatata tttttatctt gtgcaatgta catcagagat tttgagacac aagctttact
       61 ccaccgttgg cttttttccc tatcagtgat agagattgac atccctatca gtgatagaga
      121 taatgagcac agatgctgta gtgggatgtg tgtctcacct gaagagtaca aaagtccgaa
      181 acggtatcct ctacaaataa ttttgtttaa ctatggacta tgttttcaca caggaaatac
      241 caggatggca cgtaaaaccg cagcagaagc agaagaaacc cgtcagcgta ttattgatgc
      301 agcactggaa gtttttgttg cacagggtgt tagtgatgca accctggatc agattgcacg
      361 taaagccggt gttacccgtg gtgcagttta ttggcatttt aatggtaaac tggaagttct
      421 gcaggcagtt ctggcaagcc gtcagcatcc gctggaactg gattttacac cggatctggg
      481 tattgaacgt agctgggaag cagttgttgt tgcaatgctg gatgcagttc atagtccgca
      541 gagcaaacag tttagcgaaa ttctgattta tcagggtctg gatgaaagcg gtctgattca
      601 taatcgtatg gttcaggcaa gcgatcgttt tctgcagtat attcatcagg ttctgcgtca
      661 tgcagttacc cagggtgaac tgccgattaa tctggatctg cagaccagca ttggtgtttt
      721 taaaggtctg attaccggtc tgctgtatga aggtctgcgt agcaaagatc agcaggcaca
      781 gattatca
//
