LOCUS       sequence-105874-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice 
            ubiquitin promoter for transient expression in protoplast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Xie K, Minkenberg B, Yang Y
  TITLE     Boosting CRISPR/Cas9 multiplex editing capability with the 
            endogenous tRNA-processing system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 
            10.1073/pnas.1420294112. Epub 2015 Mar 2.
  PUBMED    25733849
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420294112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3570-5"
COMMENT     Sequence Label: pRGE32
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        406..481
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgccagctta ngantcttta aacatacgaa cagatcactt aaagttcttc tgaagcaact
       61 taaagttatc aggcatgcat ggatcttgga ggaatcagat gtgcagtcag ggaccatagc
      121 acaagacagg cgtcttctac tggtgctacc agcaaatgct ggaagccggg aacactgggt
      181 acgttggaaa ccacgtgatg tgaagaagta agataaactg taggagaaaa gcatttcgta
      241 gtgggccatg aagcctttca ggacatgtat tgcagtatgg gccggcccat tacgcaattg
      301 gacgacaaca aagactagta ttagtaccac ctcggctatc cacatagatc aaagctgatt
      361 taaaagagtt gtgcagatga tccgtggcag gagaccgagg tctcggtttt agagctagaa
      421 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
      481 cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg
      541 tgcatgcctg caggtccaca aattcgggtc aaggcggaag ccagcgcgcc accccacgtc
      601 agcaaatacg gaggcgcggg gttgacggcg tcacccggtc ctaacggcga ccaacaaacc
      661 agccagaaga aattacagta aaaaaaaagt aaattgcact ttgatccacc ttttattacc
      721 taagtctcaa tttggatcac ccttaaacct atcttttcaa tttgggccgg gttgtggttt
      781 ggactaccat gaacaacttt tcgtcatgtc taacttccct ttcagcaaac atatgaacca
      841 tatatagagg agatcggccg tatactagag ctgatgtgtt taaggtcgtt gattgcacga
      901 gaaaaaaaaa tccaaatcgc aacaatagca aatttatctg gttcaaagtg aaaagatatg
      961 tttaaaggta gtccaaagta aaacttatag ataataaaat gtggtccaaa gcgtaattca
     1021 ctcaaaaaaa atcaacgaga cgtgtaccaa acgganacaa acggcatctt ctcgaaattt
     1081 cccaaccgct cgctcgcccg cctcgtcttc ccggaaaccg cggnggtttc agcgt
//
